GB 5413.40-2016 National Standard of Food Safety Determination of Nucleotide in Foods and Milk Products for Infants and Young Children (English Version)
1 Scope
This standard specifies the method for determining the total amount of free nucleotide through liquid chromatography.
This standard is applicable to the determination of total amount of free nucleotide (including cytidylic acid, uridylic acid, hypoxanthine nucleotide, guanylic acid, adenylic acid) in foods and milk products for infants and young children.
2 Principle
The specimen shall be subject to water extraction; precipitant is used to precipitate the protein; separate the specimen with high performance liquid chromatography; determine the nucleotide content in the specimen by adopting external standard method with ultraviolet detector.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents, Grade III water (defined in GB/T 6682) and Grade I water (defined in GB/T 6682 and used for mobile phase of liquid chromatography) are adopted for the purposes of this method.
3.1 Reagents
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Steps
6 Expression of Analysis Result
7 Precision
8 Others
Appendix A High Performance Liquid Chromatogram for the Nucleotide Standard Product and Specimen
Standard
GB 5413.40-2016 National Standard of Food Safety Determination of Nucleotide in Foods and Milk Products for Infants and Young Children (English Version)
Standard No.
GB 5413.40-2016
Status
valid
Language
English
File Format
PDF
Word Count
2500 words
Price(USD)
50.0
Implemented on
2016-9-20
Delivery
via email in 1 business day
Detail of GB 5413.40-2016
Standard No.
GB 5413.40-2016
English Name
National Standard of Food Safety Determination of Nucleotide in Foods and Milk Products for Infants and Young Children
1 Scope
This standard specifies the method for determining the total amount of free nucleotide through liquid chromatography.
This standard is applicable to the determination of total amount of free nucleotide (including cytidylic acid, uridylic acid, hypoxanthine nucleotide, guanylic acid, adenylic acid) in foods and milk products for infants and young children.
2 Principle
The specimen shall be subject to water extraction; precipitant is used to precipitate the protein; separate the specimen with high performance liquid chromatography; determine the nucleotide content in the specimen by adopting external standard method with ultraviolet detector.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents, Grade III water (defined in GB/T 6682) and Grade I water (defined in GB/T 6682 and used for mobile phase of liquid chromatography) are adopted for the purposes of this method.
3.1 Reagents
Contents of GB 5413.40-2016
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Steps
6 Expression of Analysis Result
7 Precision
8 Others
Appendix A High Performance Liquid Chromatogram for the Nucleotide Standard Product and Specimen