Detail of GB/T 23527.1-2023

Introduction of GB/T 23527.1-2023

GB/T 23527.1-2023 Quality requirements for enzyme preparations - Part 1: Protease preparations 1 Scope This document specifies the product classification, requirements, inspection rules, marking, packaging, transportation and storage of protease preparations, and provides the test methods. This document is applicable to the production, inspection and sale of protease preparations derived from microbial fermentation or plant tissues. 2 Normative references The following documents contain provisions which, through reference in this text, constitute provisions of this document. For dated references, only the edition cited applies. For undated references, the latest edition (including any amendments) applies. GB/T 191 Packaging - Pictorial marking for handling of goods GB/T 601 Chemical reagent - Preparations of reference titration solutions GB/T 602 Chemical reagent - Preparations of standard solutions for impurity GB/T 603 Chemical reagent - Preparations of reagent solutions for use in test methods GB/T 6682 Water for analytical laboratory use - Specification and test methods QB/T 1803 General methods of determination for industrial enzymes 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 protease enzyme that catalyzes the peptide bonds within hydrolyzed protein molecules and transfers them into peptides or amino acids 3.2 protease preparations products prepared, using preparation process, etc. with extracted and purified protease as the main catalytic active component Note: Ingredients may be added to the preparation process to facilitate the storage, stability and use of the product. 3.3 activity unit of protease amount of protease required for casein hydrolysate to produce 1 μg tyrosine for 1 min under certain temperature and pH conditions, i.e., one activity unit of protease Note: It is expressed in “U”. 3.4 protease activity activity of protease preparations ability to catalyze proteolysis into polypeptide and amino acids, which is expressed as activity unit of protease contained in 1g of solid protease preparation (or 1 mL of liquid protease preparation) Note: It is expressed in U/g or U/mL. 4 Product classification 4.1 By application fields of products Protease preparations for the food industry and other industrial uses. 4.2 By product forms Protease preparations in solid and liquid dosage forms 4.3 By pH range of product action Acid, neutral and alkaline protease preparations. 5 Requirements 5.1 Sensory requirements See Table 1. Table 1 Sensory requirements

Contents of GB/T 23527.1-2023

Foreword i 1 Scope 2 Normative references 3 Terms and definitions 4 Product classification 5 Requirements 6 Test methods 7 Inspection rules 8 Marking, packaging, transportation and storage Annex A (Normative) Determination of protease activity - Folin method Annex B (Informative) Determination of protease activity - ultraviolet spectrophotometric method Annex C (Informative) Determination of alkaline protease activity - automatic biochemical analysis method Annex D (Informative) Determination of protease activity - microcolorimetric method