Detail of GB 5009.36-2023

Introduction of GB 5009.36-2023

GB 5009.36-2023 National food safety standard - Determination of cyanide in food 1 Scope This standard specifies methods for determination of cyanide in foods. Method I Spectrophotometry is applicable to the determination of cyanide in distilled alcoholic drinks and its integrated alcoholic drinks, edible alcohols, cassava powder, packaged drinking water and mineral water. Method II Gas chromatography (GC) and Method III Gas chromatography-mass spectrometry (GC-MS) are applicable to the determination of cyanide in Baijiu, edible alcohols, grains, packaged drinking water and mineral water. Method IV Ion chromatography (IC) is applicable to the determination of cyanide in distilled alcoholic drinks and its integrated alcoholic drinks, edible alcohol, packaged drinking water, mineral water and almond-based beverages. Method V Flow injection (FI)/sequential injection (SI) -spectrophotometry is applicable to the determination of cyanide in distilled alcoholic drinks and its integrated alcoholic drinks, edible alcohols, packaged drinking water and mineral water. Method I Spectrophotometry 2 Principle The specimens are either heated under alkaline conditions to remove organics with high-boiling points or heated and distilled under acidic conditions. After being dissolved or absorbed by sodium hydroxide solution, cyanide is converted into cyanogen chloride with chloramine-T at pH = 7.0, and then reacted with isonicotinic acid-pyrazolone to generate a blue color, and finally quantified by comparison with the standard series. 3 Reagents and materials Unless otherwise specified, analytical-grade reagents and Grade III water as specified in GB/T 6682 are used for the purpose of this method. 3.1 Reagents 3.1.1 Absolute ethanol(C2H6O). 3.1.2 Zinc acetate (C4H6O4Zn). 3.1.3 Tartaric acid (C4H6O6). 3.1.4 Sodium hydroxide (NaOH). 3.1.5 Monopotassium phosphate (KH2PO4). 3.1.6 Disodium phosphate (Na2HPO4). 3.1.7 Glacial acetic acid (C2H4O2): >99.5%. 3.1.8 Isonicotinic acid (C6H5O2N). 3.1.9 Pyrazolone (C10H10N2O). 3.1.10 Chloramine-T (C7H7SO2NClNa·3H2O). 3.1.11 Methyl orange (C14H14O3N3SNa). 3.1.12 Phenolphthalein (C20H14O4). 3.2 Preparation of reagents

Contents of GB 5009.36-2023

Foreword i 1 Scope Method I Spectrophotometry 2 Principle 3 Reagents and materials 4 Instruments and apparatus 5 Analysis procedure 6 Expression of analysis results 7 Accuracy 8 Miscellaneous Method II Gas chromatography 9 Principle 10 Reagents and materials 11 Instruments and apparatus 12 Analysis procedure 13 Expression of analysis results 14 Accuracy 15 Miscellaneous Method III Gas chromatography-mass spectrometry 16 Principle 17 Reagents and materials 18 Instruments and apparatus 19 Analysis procedure 20 Expression of analysis results 21 Accuracy 22 Miscellaneous Method IV Ion chromatography 23 Principle 24 Reagents and materials 25 Instruments and apparatus 26 Analysis procedure 27 Expression of analysis results 28 Accuracy 29 Miscellaneous Method V Flow injection/sequential injection-spectrophotometry 30 Principle 31 Reagents and materials 32 Instruments and apparatus 33 Analysis procedure 34 Expression of analysis results 35 Accuracy 36 Miscellaneous Annex A Reference conditions for headspace analysis Annex B Standard gas chromatogram of cyanogen chloride Annex C Setting of ion chromatography conditions and examples of standard chromatogram Annex D Determination spectrum of cyanide ion in distilled alcoholic drinks and its integrated alcoholic drinks by flow injection/sequential injection