Standard No.:	GB/T 17480-2008
English Name:	Determination of aflatoxin B1 in animal feeding stuffs - Enzyme-linked immunosorbent assay
Chinese Name:	饲料中黄曲霉毒素B1的测定　酶联免疫吸附法
Chinese Classification:	B46    Livestock and poultry feed and additive
Professional Classification:	GB    National Standard
Issued by:	AQSIQ SAC
Issued on:	1998-8-28
Implemented on:	2009-2-1
Status:	valid
Superseding:	GB/T 17480-1998 Determination of aflatoxin B1 in feeds--Enzyme-linked immunosorbent assay
Language:	English
File Format:	PDF
Word Count:	4000 words
Price(USD):	100.0
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1 Scope This standard specifies the enzyme-linked immunosorbent assay (ELISA) method for the determination of aflatoxin B1 in animal feeding stuffs. This standard is applicable to the determination of aflatoxin B1 in various feed materials, formula feeds and concentrate feeds. The detection limit of this standard is 0.1μg/kg. 2 Normative Reference The following standard contains provisions which, through reference in this standard, constitute provisions of this standard. For dated reference, subsequent amendments to (excluding correction to), or revisions of, any of these publications do not apply. However, all parties coming to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the standards. For undated references, the latest edition of the normative document referred to applies. GB/T 20195 Animal Feeding Stuffs - Preparation of Test Samples (GB/T 20195-2006, ISO 6498:1998, IDT) 3 Principle

Foreword I 1 Scope 2 Normative Reference 3 Principle 4 Reagents and Materials 5 Apparatus and Equipment 6 Analysis Procedure 7 Precision Appendix A (Informative) Extraction Method for Concentrate Feed Appendix B (Informative) Specimen Solution Dilution