Standard No.:	SN/T 2154-2008
English Name:	Determination of coagulase - Positive staphylococci in import and export food - Technique using rabbit plasma fibrinogen agar medium
Chinese Name:	进出口食品中凝固酶阳性葡萄球菌检测方法 兔血浆纤维蛋白原琼脂培养基技术
Professional Classification:	SN    Professional Standard - Import&Export Inspection
Issued by:	AQSIQ
Issued on:	2008-9-4
Implemented on:	2009-3-16
Status:	superseded
Superseded by:	GB 4789.10-2016 National Food Safety Standard—Food Microbiological Examination—Examination of Staphylococcus Aureus
Superseded on:	2017-6-23
Abolished on:	2017-06-23
Language:	English
File Format:	PDF
Word Count:	3000 words
Price(USD):	374.0
Delivery:	via email in 1 business day

1 Scope This standard specifies the determination of coagulase-positive staphylococci in import and export food In this standard, the direct plate count method is applicable to counting the coagulase-positive staphylococci in food, and the MPN method is applicable to food with lower coagulase-positive staphylococci and higher infectious microbe. The foods such as animal food etc. may be applied. 2 Equipment and Materials 2.1 Sterilizing equipment: the high-pressure steam sterilization pot and dry heat sterilization box. 2.2 Water bath:48℃±1℃. 2.3 Incubator:36℃±1℃. 2.4 Pipette: 1ml and 10ml, respectively with the division of 0.01ml and 0.1ml. 2.5 Inoculating loop: in the diameter of 3mm. 2.6 Scale: the range of 2kg and the sensitivity of 0.1g.

Foreword I 1 Scope 3 Culture Media and Reagents 4 Inspection Procedures 5 Operation Procedures 6 Report of Total Colony Number of Coagulase-positive Staphylococci 7 Inspection Procedures 8 Operation Procedures 9 Report Appendix A (Normative) Culture Media and Reagents Appendix B (Normative) Most Probable Number (MNP) Table in 1g(ml) of Examination Samples