General Administration of Quality Supervision, Inspection and Quarantine
Issued on:
2016-12-12
Implemented on:
2017-7-1
Status:
valid
Target Language:
English
File Format:
PDF
Word Count:
3500 words
Translation Price(USD):
120.0
Delivery:
via email in 1 business day
SN/T 4677-2016 Determination of azodicarbonamide in foods for export - HPLC method
1 Scope
This standard specifies the method for the determination of azodicarbonamide in flour for export by high performance liquid chromatography (HPLC).
This standard is applicable to the determination of azodicarbonamide in flour.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition (including any amendments) applies.
GB/T 6682 Water for analytical laboratory use - Specification and test methods
3 Method summary
After being extracted ultrasonically in acetone, degreased, and detected by high performance liquid chromatography-diode array detector, the samples are quantified by external standard method of matrix-matched standard working curve.
4 Reagents and materials
Unless otherwise specified, analytically-pure reagents and Class I water defined in GB/T 6682 shall be adopted.
4.1 Acetone: chromatographically pure.
4.2 n-hexane: chromatographically pure.
4.3 Phosphoric acid.
4.4 Dipotassium hydrogen phosphate.
4.5 0.02% dipotassium hydrogen phosphate solution: accurately weigh 0.2g dipotassium hydrogen phosphate, dissolve it with water and dilute to 1L, and then adjust it to pH=2.4 with phosphoric acid (4.3).
4.6 0.5% dipotassium hydrogen phosphate solution: accurately weigh 0.5g dipotassium hydrogen phosphate, dissolve it with water and dilute to 100mL.
4.7 Azodicarbonamide standard material: CAS No.: 123-77-3, molecular formula: C2H4N4O2, relative molecular mass: 116.1.
Contents
Foreword i
1 Scope
2 Normative references
3 Method summary
4 Reagents and materials
5 Instruments and apparatus
6 Analysis steps
7 Calculation and expression of results
8 Limit of quantification and recovery rate
Annex A (Informative) High performance liquid chromatogram of azodicarbonamide standard material
SN/T 4677-2016 Determination of azodicarbonamide in foods for export―HPLC method (English Version)
Standard No.
SN/T 4677-2016
Status
valid
Language
English
File Format
PDF
Word Count
3500 words
Price(USD)
120.0
Implemented on
2017-7-1
Delivery
via email in 1 business day
Detail of SN/T 4677-2016
Standard No.
SN/T 4677-2016
English Name
Determination of azodicarbonamide in foods for export―HPLC method
Chinese Name
出口食品中偶氮甲酰胺的测定方法高效液相色谱法
Chinese Classification
Professional Classification
SN
ICS Classification
Issued by
General Administration of Quality Supervision, Inspection and Quarantine
Issued on
2016-12-12
Implemented on
2017-7-1
Status
valid
Superseded by
Superseded on
Abolished on
Superseding
Language
English
File Format
PDF
Word Count
3500 words
Price(USD)
120.0
Keywords
SN/T 4677-2016, SN 4677-2016, SNT 4677-2016, SN/T4677-2016, SN/T 4677, SN/T4677, SN4677-2016, SN 4677, SN4677, SNT4677-2016, SNT 4677, SNT4677
Introduction of SN/T 4677-2016
SN/T 4677-2016 Determination of azodicarbonamide in foods for export - HPLC method
1 Scope
This standard specifies the method for the determination of azodicarbonamide in flour for export by high performance liquid chromatography (HPLC).
This standard is applicable to the determination of azodicarbonamide in flour.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition (including any amendments) applies.
GB/T 6682 Water for analytical laboratory use - Specification and test methods
3 Method summary
After being extracted ultrasonically in acetone, degreased, and detected by high performance liquid chromatography-diode array detector, the samples are quantified by external standard method of matrix-matched standard working curve.
4 Reagents and materials
Unless otherwise specified, analytically-pure reagents and Class I water defined in GB/T 6682 shall be adopted.
4.1 Acetone: chromatographically pure.
4.2 n-hexane: chromatographically pure.
4.3 Phosphoric acid.
4.4 Dipotassium hydrogen phosphate.
4.5 0.02% dipotassium hydrogen phosphate solution: accurately weigh 0.2g dipotassium hydrogen phosphate, dissolve it with water and dilute to 1L, and then adjust it to pH=2.4 with phosphoric acid (4.3).
4.6 0.5% dipotassium hydrogen phosphate solution: accurately weigh 0.5g dipotassium hydrogen phosphate, dissolve it with water and dilute to 100mL.
4.7 Azodicarbonamide standard material: CAS No.: 123-77-3, molecular formula: C2H4N4O2, relative molecular mass: 116.1.
Contents of SN/T 4677-2016
Contents
Foreword i
1 Scope
2 Normative references
3 Method summary
4 Reagents and materials
5 Instruments and apparatus
6 Analysis steps
7 Calculation and expression of results
8 Limit of quantification and recovery rate
Annex A (Informative) High performance liquid chromatogram of azodicarbonamide standard material
