1 Scope
This standard specifies determination methods for trimethylamine in aquatic animals and their products as well as meat and meat products.
This standard is applicable to the determination of trimethylamine in the aquatic animals and their products as well as meat and meat products.
Method I Headspace Gas Chromatography - Mass Spectrometry
2 Principle
Extract the specimen with 5% trichloroacetic acid solution, put the extract into a sealed headspace bottle, transform the trimethylamine hydrochloride to trimethylamine under the action of alkali liquor, balance at 40℃ for 40 min, so that the trimethylamine realizes dynamic balance in gas-liquid phases. Pipet the gas in the headspace bottle and inject into the gas chromatography - mass spectrometer for detection, carry out the qualitative determination by retention time (RT), assisted qualitative ion (m/z 59 and m/z 42) and quantitative ion (m/z 58) and the quantitative determination by external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Class-I water (defined in GB/T 6682) are adopted for the purpose of this method.
3.1 Reagents
3.1.1 Sodium hydroxide (NaOH)
3.1.2 Trichloroacetic acid (C2HCl3O2).
3.2 Preparation of reagents
3.2.1 50% sodium hydroxide solution: weigh 100 g sodium hydroxide and dissolve in 100mL water (20℃~30℃).
3.2.2 5% trichloroacetic acid solution: weigh 25 g trichloroacetic acid, dissolve in water and scale the volume to 500 mL.
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Steps
6 Expression of Analysis Results
7 Accuracy
8 Other
9 Principle
10 Reagents and Materials
11 Instruments and Apparatus
12 Analysis Steps
13 Expression of Analysis Results
14 Accuracy
15 Other
Annex A Chromatogram and Mass Spectrum
Standard
GB 5009.179-2016 National Food Safety Standard - Determination of trimethylamine in food (English Version)
Standard No.
GB 5009.179-2016
Status
valid
Language
English
File Format
PDF
Word Count
3000 words
Price(USD)
80.0
Implemented on
2017-3-1
Delivery
via email in 1 business day
Detail of GB 5009.179-2016
Standard No.
GB 5009.179-2016
English Name
National Food Safety Standard - Determination of trimethylamine in food
1 Scope
This standard specifies determination methods for trimethylamine in aquatic animals and their products as well as meat and meat products.
This standard is applicable to the determination of trimethylamine in the aquatic animals and their products as well as meat and meat products.
Method I Headspace Gas Chromatography - Mass Spectrometry
2 Principle
Extract the specimen with 5% trichloroacetic acid solution, put the extract into a sealed headspace bottle, transform the trimethylamine hydrochloride to trimethylamine under the action of alkali liquor, balance at 40℃ for 40 min, so that the trimethylamine realizes dynamic balance in gas-liquid phases. Pipet the gas in the headspace bottle and inject into the gas chromatography - mass spectrometer for detection, carry out the qualitative determination by retention time (RT), assisted qualitative ion (m/z 59 and m/z 42) and quantitative ion (m/z 58) and the quantitative determination by external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Class-I water (defined in GB/T 6682) are adopted for the purpose of this method.
3.1 Reagents
3.1.1 Sodium hydroxide (NaOH)
3.1.2 Trichloroacetic acid (C2HCl3O2).
3.2 Preparation of reagents
3.2.1 50% sodium hydroxide solution: weigh 100 g sodium hydroxide and dissolve in 100mL water (20℃~30℃).
3.2.2 5% trichloroacetic acid solution: weigh 25 g trichloroacetic acid, dissolve in water and scale the volume to 500 mL.
Contents of GB 5009.179-2016
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Steps
6 Expression of Analysis Results
7 Accuracy
8 Other
9 Principle
10 Reagents and Materials
11 Instruments and Apparatus
12 Analysis Steps
13 Expression of Analysis Results
14 Accuracy
15 Other
Annex A Chromatogram and Mass Spectrum