Standard No.:	GB/T 20758-2006
English Name:	Method for the determination of testosterone epi-testosterone and progesterone residues in bovine liver and muscle tissues - LC-MS-MS method
Chinese Name:	牛肝和牛肉中睾酮、表睾酮、孕酮残留量的测定 液相色谱-串联质谱法
Chinese Classification:	X04    Basic standards and general methods
Professional Classification:	GB    National Standard
Source Content Issued by:	AQSIQ; SAC
Issued on:	2006-12-31
Implemented on:	2007-3-1
Status:	valid
Target Language:	English
File Format:	PDF
Word Count:	4000 words
Translation Price(USD):	100.0
Delivery:	via email in 1 business day

1 Scope This standard specified the determination of testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues by LC-MS-MS method. This standard is applicable to the determination of testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues. Method detection limits of this standard: in bovine liver, testosterone, epi-testosterone and progesterone: 0.5 μg/kg; in muscle tissues, testosterone and epi-testosterone: 0.1 μg/kg, and progesterone: 0.5 μg/kg. 2 Normative references The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this standard. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition applies. GB/T 6379.1 Accuracy (trueness and precision) of measurement methods and results—Part 1: General principles and definitions ( GB/T 6379.1-2004, ISO 5725-1:1994, IDT) GB/T 6379.2 Accuracy (trueness and precision) of measurement methods and results—Part 2: Basic method for the determination of repeatability and reproducibility of a Standard measurement method (GB/T 6379.2-2004, ISO 5725-2:1994, IDT) GB/T 6682 Water for analytical laboratory use-specification and test methods (GB/T 6682-1992, neq ISO 3696: 1987) 3 Principle After enzymolysis, the testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues are extracted with methanol and methyl tert-butyl ether; the extracting solution is purified with C18 solid phase extraction column, while the liver sample is purified again with silica gel solid phase extraction column; the eluent is concentrated to constant volume for determination by LC-MS-MS method.

Foreword i 1 Scope 2 Normative references 3 Principle 4 Reagents and materials 5 Apparatus 6 Preparation and preservation of specimen 7 Determination procedures 8 Calculation and expression of results 9 Precision Annex A (Informative) Multiple reaction monitoring (MRM) chromatograms of testosterone, epi-testosterone and progesterone Annex B (Informative) Recovery rate