GB/T 20758-2006 Method for the determination of testosterone epi-testosterone and progesterone residues in bovine liver and muscle tissues - LC-MS-MS method (English Version)
This standard specified the determination of testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues by LC-MS-MS method.
This standard is applicable to the determination of testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues.
Method detection limits of this standard: in bovine liver, testosterone, epi-testosterone and progesterone: 0.5 μg/kg; in muscle tissues, testosterone and epi-testosterone: 0.1 μg/kg, and progesterone: 0.5 μg/kg.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this standard. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition applies.
GB/T 6379.1 Accuracy (trueness and precision) of measurement methods and results—Part 1: General principles and definitions ( GB/T 6379.1-2004, ISO 5725-1:1994, IDT)
GB/T 6379.2 Accuracy (trueness and precision) of measurement methods and results—Part 2: Basic method for the determination of repeatability and reproducibility of a Standard measurement method (GB/T 6379.2-2004, ISO 5725-2:1994, IDT)
GB/T 6682 Water for analytical laboratory use-specification and test methods (GB/T 6682-1992, neq ISO 3696: 1987)
3 Principle
After enzymolysis, the testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues are extracted with methanol and methyl tert-butyl ether; the extracting solution is purified with C18 solid phase extraction column, while the liver sample is purified again with silica gel solid phase extraction column; the eluent is concentrated to constant volume for determination by LC-MS-MS method.
Foreword i 1 Scope 2 Normative references 3 Principle 4 Reagents and materials 5 Apparatus 6 Preparation and preservation of specimen 7 Determination procedures 8 Calculation and expression of results 9 Precision Annex A (Informative) Multiple reaction monitoring (MRM) chromatograms of testosterone, epi-testosterone and progesterone Annex B (Informative) Recovery rate
Standard
GB/T 20758-2006 Method for the determination of testosterone epi-testosterone and progesterone residues in bovine liver and muscle tissues - LC-MS-MS method (English Version)
Standard No.
GB/T 20758-2006
Status
valid
Language
English
File Format
PDF
Word Count
4000 words
Price(USD)
100.0
Implemented on
2007-3-1
Delivery
via email in 1 business day
Detail of GB/T 20758-2006
Standard No.
GB/T 20758-2006
English Name
Method for the determination of testosterone epi-testosterone and progesterone residues in bovine liver and muscle tissues - LC-MS-MS method
1 Scope
This standard specified the determination of testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues by LC-MS-MS method.
This standard is applicable to the determination of testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues.
Method detection limits of this standard: in bovine liver, testosterone, epi-testosterone and progesterone: 0.5 μg/kg; in muscle tissues, testosterone and epi-testosterone: 0.1 μg/kg, and progesterone: 0.5 μg/kg.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this standard. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition applies.
GB/T 6379.1 Accuracy (trueness and precision) of measurement methods and results—Part 1: General principles and definitions ( GB/T 6379.1-2004, ISO 5725-1:1994, IDT)
GB/T 6379.2 Accuracy (trueness and precision) of measurement methods and results—Part 2: Basic method for the determination of repeatability and reproducibility of a Standard measurement method (GB/T 6379.2-2004, ISO 5725-2:1994, IDT)
GB/T 6682 Water for analytical laboratory use-specification and test methods (GB/T 6682-1992, neq ISO 3696: 1987)
3 Principle
After enzymolysis, the testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues are extracted with methanol and methyl tert-butyl ether; the extracting solution is purified with C18 solid phase extraction column, while the liver sample is purified again with silica gel solid phase extraction column; the eluent is concentrated to constant volume for determination by LC-MS-MS method.
Contents of GB/T 20758-2006
Foreword i
1 Scope
2 Normative references
3 Principle
4 Reagents and materials
5 Apparatus
6 Preparation and preservation of specimen
7 Determination procedures
8 Calculation and expression of results
9 Precision
Annex A (Informative) Multiple reaction monitoring (MRM) chromatograms of testosterone, epi-testosterone and progesterone
Annex B (Informative) Recovery rate
