1 Scope
This standard specifies spectrophotometric method for the determination of ascorbic acid in foods.
It is applicable to the determination of reductive-form ascorbic acid in foods.
It is not applicable to the determination of dehydrogenation-form ascorbic acid.
2 Principle
In acetic acid solution, the reaction of ascorbic acid with the fast blue B salt will produce yellow derivatives of oxalyl dihydrazide-2-hydroxy butyl ester. Carry out determination of absorbance at the maximum absorption wavelength of 420nm, and compare the quantitative measurement with reference series.
3 Reagent
Standard
GB/T 5009.159-2003 Determination of reductive-form ascorbic acid in foods (English Version)
Standard No.
GB/T 5009.159-2003
Status
superseded
Language
English
File Format
PDF
Word Count
1500 words
Price(USD)
184.0
Implemented on
2004-1-1
Delivery
via email in 1 business day
Detail of GB/T 5009.159-2003
Standard No.
GB/T 5009.159-2003
English Name
Determination of reductive-form ascorbic acid in foods
1 Scope
This standard specifies spectrophotometric method for the determination of ascorbic acid in foods.
It is applicable to the determination of reductive-form ascorbic acid in foods.
It is not applicable to the determination of dehydrogenation-form ascorbic acid.
2 Principle
In acetic acid solution, the reaction of ascorbic acid with the fast blue B salt will produce yellow derivatives of oxalyl dihydrazide-2-hydroxy butyl ester. Carry out determination of absorbance at the maximum absorption wavelength of 420nm, and compare the quantitative measurement with reference series.
3 Reagent
