Standard No.:	GB/T 8538-2008
English Name:	Methods for examination of drinking natural mineral water
Chinese Name:	饮用天然矿泉水检验方法
Chinese Classification:	X51    Beverage products
Professional Classification:	GB    National Standard
ICS Classification:	67.160.20 67.160.20    Non-alcoholic beverages 67.160.20
Issued by:	AQSIQ; SAC
Issued on:	2008-6-30
Implemented on:	2009-4-1
Status:	superseded
Superseded by:	GB 8538-2016 National Food Safety Standard - Methods for examination of drinking natural mineral water GB 5009.36-2016 Method for analysis of hygienic standard of grains
Superseded on:	2017-6-23
Superseding:	GB/T 8538-1995 Methods for examination of drinking natural mineral water
Language:	English
File Format:	PDF
Word Count:	900,000 words
Price(USD):	4860.0
Delivery:	via email in 1~3 business day

4.52 Coliform bacteria 4.52.1 Multiple-tube fermentation (MTF) 4.52.1.1 Measurement range This method is applicable to determination of coliform bacteria. 4.52.1.2 Principle According to the biological characteristics of coliform bacteria, like negative Gram sporeless bacterium, the cultivation of 24h at 37℃ fermenting lactose and generating gas; inoculating water sample of different amounts into culture medium containing lactose; the positive reaction after cultivation can indicate the MPN of coliform bacteria in the original water samples. 4.52.1.3 Culture medium and reagent 4.52.1.3.1 Lactose bile salt fermenting culture solution 4.52.1.3.1.1 Ingredients Single-fold Two-fold Peptone 20.0 g 40.0 g Pig bile salts (or cattle, sheep bile salts) 5.0 g 10.0 g Lactose 10.0 g 20.0 g Bromcresol purple solution (4g/L) 2.5 mL 5.0 mL Distilled water 1 000 mL 1 000 mL 4.52.1.3.1.2 Preparation: dissolve peptone, bile salt and lactose in distilled water, regulate pH value to 7.4, add bromcresol purple solution and mix; shift 10mL into test tubes separately (each test tube has a small inverted tube). Sterilize at 115℃ for 20min. 4.52.1.3.2 Brilliant green lactose bile salt culture solution (BGB) 4.52.1.3.2.1 Ingredients Peptone 10.0 g Lactose 10.0 g Bile salt 2.0 g Brilliant green, BGB 0.011 3g Distilled water 1 000 mL 4.52.1.3.2.2 Preparation: besides brilliant green, add dissolve ingredients into distilled water, regulate pH value to 7.2, add brilliant green and mix; shift 10mL into test tubes separately (each test tube has a small inverted tube); and sterilize at 115℃ for 20min. 4.52.1.4 Apparatus 4.52.1.4.1 Test tube: 150mm×20mm, 140mm×15mm. 4.52.1.4.2 Small inverted tube. 4.52.1.4.3 Quantitive portioner. 4.52.1.4.4 High-pressure sterilizer 4.52.1.4.5 Incubator: 36℃±1℃. 4.52.1.4.6 Refrigerator: 0℃~8℃. 4.52.1.4.7 Balance. 4.52.1.4.8 Microscope. 4.52.1.4.9 Electric oven. 4.52.1.5 Procedure 4.52.1.5.1 The procedures are as shown in Figure 10.

Foreword 1 Scope 2 Normative References 3 Terms and Definitions 4 Method 4.1 General 4.2 Collection and Preservation 4.3 Chroma 4.4 Odor and Taste 4.5 Visible 4.6 Turbidity 4.7 pH 4.8 Total Dissolved Solids 4.8.1 105℃ Drying-Weighting Method 4.8.2 180℃ Drying-Weighting Method 4.9 Total Hardness 4.10 Total Alkalinity 4.11 Total Acidity 4.12 Potassium and Sodium 4.12.1 Flame Emission Spectrophotometry 4.12.2 Flame Atomic Absorption Spectrophotometry 4.12.3 Ion Chromatography 4.13 Calcium 4.13.1 EDTA Titration 4.13.2 Flame Atomic Absorption Spectrophotometry 4.14 Magnesium 4.14.1 EDTA Titration 4.14.2 Flame Atomic Absorption Spectrophotometry 4.15 Iron 4.15.1 Flame Atomic Absorption Spectrophotometry 4.15.2 Naphthisodiazine Spectrophotometry 4.16 Manganese 4.16.1 Flame Atomic Absorption Spectrophotometry 4.16.2 Ammonium Persulfate Spectrophotometry 4.16.3 Formaldoxime Spectrophotometry 4.17 Copper 4.17.1 Flame Atomic Absorption Spectrophotometry 4.17.1.1 Direct Method 4.17.1.2 Extraction Method 4.17.1.3 Coprecipitation Method 4.17.1.4 Sulfhydryl Cotton Concentration Method 4.17.2 Flameless Atomic Absorption Spectrophotometry 4.17.3 Sodium Diethyldithiocarbamate Spectrophotometry 4.18 Zinc 4.18.1 Flame Atomic Absorption Spectrophotometry 4.18.2 Zinc Reagent-Cyclohexanone Spectrophotometry 4.18.3 Catalytic Oscillopolarography 4.19 Total Chromium 4.20 Lead 4.20.1 Flame Atomic Absorption Spectrophotometry 4.20.2 Flameless Atomic Absorption Spectrophotometry 4.20.3 Catalytic Oscillopolarography 4.21 Cadmium 4.21.1 Flame Atomic Absorption Spectrophotometry 4.21.2 Flameless Atomic Absorption Spectrophotometry 4.21.3 Catalytic Oscillopolarography 4.22 Mercury 4.22.1 Cold Vapor Atomic Absorption Method 4.22.2 Atomic Fluorescence Spectrometry 4.23 Silver 4.23.1 Flameless Atomic Absorption Spectrophotometry 4.23.2 Sulfhydryl Cotton Concentration-Potassium Periodate Spectrophotometry 4.24 Strontium 4.24.1 EDTA-Flame Atomic Absorption Spectrophotometry 4.24.2 High-concentration Lanthanum-Flame Atomic Absorption Spectrophotometry 4.24.3 Flame Emission Spectrometry 4.25 Lithium 4.25.1 Flame Emission Spectrometry 4.25.2 Flame Atomic Absorption Spectrophotometry 4.25.3 Ion Chromatography 4.26 Barium 4.27 Vanadium 4.27.1 Flameless Atomic Absorption Spectrophotometry 4.27.2 Catalytic Polarography 4.27.3 Gallic Acid Catalytic Spectrophotometry 4.28 Stibium 4.28.1 Hydride Atomic Fluorescence Spectrometry 4.28.2 Hydride Generation Atomic Absorption Spectrophotometry 4.29 Cobalt 4.29.1 Nitroso-R Spectrophotometry 4.29.2 Flame Atomic Absorption Spectrophotometry 4.29.3 Flameless Atomic Absorption Spectrophotometry 4.30 Nickel 4.30.1 Flame Atomic Absorption Spectrophotometry 4.30.2 Flameless Atomic Absorption Spectrophotometry 4.31 Aluminium 4.31.1 Chrome Azurol S Spectrophotometry 4.31.2 Aluminon Spectrophotometry 4.31.3 Flameless Atomic Absorption Spectrophotometry 4.32 Selenium 4.32.1 Diamine Naphthalene Fluorescence Method 4.32.2 Hydride Generation Atomic Absorption Spectrometry 4.32.3 Hydride Atomic Fluorescence Spectrometry 4.33 Arsenic 4.33.1 Silver Diethyldithiocarbamate Spectrophotometry 4.33.2 Zinc-Sulfuric Acid System New Silver Salt Spectrophotometry 4.33.3 Catalytic Oscillopolarography 4.33.4 Hydride Generation Atomic Fluorescence Spectrometry 4.34 Borate 4.34.1 Azomethine-H Spectrophotometry 4.34.2 Extraction-Curcumin Spectrophotometry 4.34.3 Curcumin Spectrophotometry 4.35 Metasillicio Acid 4.35.1 Silicon-Molybdenum Yellow Spectrophotometry 4.35.2 Silicon-Molybdenum Blue Spectrophotometry 4.36 Fluoride 4.36.1 Ion Selective Electrode Method 4.36.2 Fluor Reagent Dualwavelength Spectrophotometry 4.36.3 Fluor Reagent Spectrophotometry 4.36.4 Ion Chromatography 4.37 Chloride 4.37.1 Silver Nitrate Volumetric Method 4.37.2 Ion Chromatography 4.38 Iodide 4.38.1 Catalytic Reductive Spectrophotometry 4.38.2 Gas Chromatography 4.38.3 Ion Chromatography 4.38.4 High-concentration Iodide Colorimetric Method 4.39 Carbon Dioxide 4.40 Nitrate 4.40.1 Thymol Spectrophotometry 4.40.2 Ion Chromatography 4.40.3 UV Spectrophotometry 4.41 Nitrite 4.42 Carbonate and Bicarbonate 4.43 Sulfate 4.43.1 EDTA Titration 4.43.2 Barium Chromate Colorimetric Method 4.43.3 Barium Sulfate Turbidimetric Method 4.43.4 Ion Chromatography 4.44 Oxygen Consumption 4.44.1 Acidic Potassium Permanganate Titration 4.44.2 Alkaline Potassium Permanganate Titration 4.45 Cyanide 4.45.1 Isonicotinic Acid-Pyrazolone Spectrophotometry 4.45.2 Isonicotinic Acid-Barbituric Acid Spectrophotometry 4.45.3 Flow Injection Online Distillation Method 4.46 Volatile Phenolic Compounds 4.46.1 4-aminoantipyrine Trichloromethane Extraction Spectrophotometry 4.46.2 Flow Injection Online Distillation Method 4.47 Anion Synthetic Detergent 4.47.1 Methylene Blue Spectrophotometry 4.47.2 Naphthisodiazine Extraction Spectrophotometry 4.48 Mineral Oil 4.48.1 Infrared Spectrophotometry 4.48.2 Non-dispersive Infrared Spectrophotometry 4.48.3 Weighing Method 4.48.4 UV Spectrophotometry 4.49 Bromate 4.49.1 Ion Chromatography (Hydroxyl System Leacheate) 4.49.2 Ion Chromatography (Carbonate System Leacheate) 4.50 Total β Radioactivity 4.50.1 Thin-film Method 4.50.2 Active Carbon Adsorption Method 4.51 Radium-266 Radioactivity 4.52 Coliform Bacteria 4.52.1 Multiple-tube Fermentation Method 4.52.2 Filter Membrane Method 4.53 Streptococcus Faecalis 4.54 Pseudomonas Aeruginosa 4.55 Clostridium Perfringens Appendix A (Informative) Methods for Examination of Various Elements in Drinking Natural Mineral Water - Inductively Coupled Plasma-Optical Emission Spectrometry and Inductively Coupled Plasma-Mass Spectrometry Appendix B (Informative) Methods for Examination of Sulfide, Phosphate, Tritium and Aerobic Bacteria Count in Drinking Natural Mineral Water