Standard No.:	NY/T 2293.2-2012
English Name:	Bacterial Pesticides - Bacillus Subtilis - Part 2: Bacillus Subtilis Wettable Powders (WP)
Chinese Name:	细菌微生物农药 枯草芽孢杆菌 第2部分:枯草芽孢杆菌可湿性粉剂
Professional Classification:	NY    Professional Standard - Agriculture
Issued by:	Ministry of Agriculture
Issued on:	2012-12-24
Implemented on:	2013-3-1
Status:	valid
Language:	English
File Format:	PDF
Word Count:	6000 words
Price(USD):	100.0
Delivery:	via email in 1 business day

Bacterial pesticides - Bacillus subtilis - Part 2: Bacillus subtilis wettable powders (WP) 1 Scope This part specifies the requirements, test methods, inspection and acceptance, marking, labeling, packaging, storage and transportation of bacterial pesticides of Bacillus subtilis wettable powders (WP). This part is applicable to the bacillus subtilis wettable powders, which is made of bacillus subtilis technical concentrates with spores as the main component and added with appropriate additives and fillers. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. GB/T 191 Packaging - Pictorial marking for handling of goods GB/T 1601 Determination method of pH value for pesticides GB/T 1604 Commodity pesticide regulations for acceptance GB/T 1605 Sampling method for commodity pesticides GB 3796 General rule for packing of pesticides GB/T 5451 Testing method for the wettability of dispersible powders of pesticides GB/T 8170-2008 Rules of rounding off for numerical values & expression and judgment of limiting values GB/T 14825 Determination method of suspensibility for pesticides GB/T l6150-1995 Sieve test for dustable and wettable powders of pesticides NY/T 2293.1-2012 Bacterial pesticides-Bacillus cereus - Part 1:Bacillus subtilis technical concentrates (TK) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 Bacillus subtilis wettable powders (WP) powdery preparation made by Bacillus subtilis technical concentrates and appropriate additives and fillers, which may be dispersed in water to form a stable suspension solution 3.2 colony forming units (CFU) dilute Bacillus subtilis wettable powder with water to obtain bacterial solution, disperse its single spore on agar plate through coating. After being cultured, every viable spore forms a colony, that is, a colony forming unit (CFU), and calculate the viable spore density in a unit microbial pesticide sample by observing the number of colonies with naked eyes 3.3 number of microbial contamination sum of the number of microbial colonies except Bacillus subtilis colony in the Bacillus subtilis wettable powder 3.4 frequency of microbial contamination percentage rate of the amount of other fungi and bacteria except Bacillus subtilis in the Bacillus subtilis wettable powder in the total bacteria amount 3.5 storage stability relative percentage ratio of the number of viable fungal of the Bacillus subtilis wettable powder to its indicated value after storage at room temperature and (or) below room temperature for a certain period of time

Foreword i 1 Scope 2 Normative references 3 Terms and definitions 4 Requirements 5 Test methods 6 Product inspection and acceptance 7 Marking, labeling, packaging, storage and transportation Annex A (Informative) Description of active ingredient