Detail of GB 5009.22-2016

Introduction of GB 5009.22-2016

1 Scope This standard specifies the determination methods of aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2 (hereinafter referred to as AFT B1, AFT B2, AFT G1 and AFT G2) in foods. Method I in this standard is isotope-dilution liquid chromatography-tandem mass spectrometry, which is applicable to the determination of AFT B1, AFT B2, AFT G1 and AFT G2 in cereals and their products, beans and their products, nuts and seeds, oils and their products, condiments, formula foods for infants and young children and complementary foods for infants and young children. Method II in this standard is high performance liquid chromatography-pre-column derivatization method, which is applicable to the determination of AFT B1, AFT B2, AFT G1 and AFT G2 in cereals and their products, beans and their products, nuts and seeds, oils and their products, condiments, formula foods for infants and young children and complementary foods for infants and young children. Method III in this standard is high performance liquid chromatography - post-column derivatization method, which is applicable to the determination of AFT B1, AFT B2, AFT G1 and AFT G2 in cereals and their products, beans and their products, nuts and seeds, oils and their products, condiments, formula foods for infants and young children and complementary foods for infants and young children. Method IV in this standard is enzyme-linked immunosorbent screening method, which is applicable to the determination of AFT B1 in cereals and their products, beans and their products, nuts and seeds, oils and their products, condiments, formula foods for infants and young children and complementary foods for infants and young children. Method V in this standard is thin layer chromatography, which is applicable to the determination of AFT B1 in cereals and their products, beans and their products, nuts and seeds, oils and their products and condiments. Method I Isotope-dilution Liquid Chromatography-tandem Mass Spectrometry 2 Principle Extract the aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2 in the specimen with acetonitrile-water solution or methanol-water solution, dilute the extracting solution with phosphate buffer solution containing 1% TritonX-100 (or Tween-20) (where necessary, preliminary purification by aflatoxin solid-phase purification column shall be carried out), purify and enrich through immunoaffinity column, concentrate the purified solution, scale the volume, separate with liquid chromatograph after filtration, examine with tandem mass spectrum and quantify with isotope internal standard method. 3 Reagents and Materials Unless otherwise specified, analytically-pure reagents and Class-I water (defined in GB/T 6682) are adopted for the purpose of this method. 3.1 Reagents 3.1.1 Acetonitrile (CH3CN): chromatographically pure. 3.1.2 Methanol (CH3OH): chromatographically pure. 3.1.3 Ammonium acetate (CH3COONH4): chromatographically pure. 3.1.4 Sodium chloride (NaCl). 3.1.5 Disodium hydrogen phosphate: (Na2HPO4).

Contents of GB 5009.22-2016

Foreword i 1 Scope 2 Principle 3 Reagents and Materials 4 Instruments and Apparatus 5 Analysis Steps 6 Expression of Analysis Results 7 Accuracy 8 Other 9 Principle 10 Reagents and Materials 11 Instruments and Apparatus 12 Analysis Steps 13 Expression of Analysis Results 14 Accuracy 15 Other 16 Principle 17 Reagents and Materials 18 Instruments and Apparatus 19 Analysis Steps 20 Expression of Analysis Results 21 Accuracy 22 Other 23 Principle 24 Reagents and Materials 25 Instruments and Apparatus 26 Analysis Steps 27 Expression of Analysis Results 28 Accuracy 29 Other 30 Principle 31 Reagents and Materials 32 Instruments and Apparatus 33 Analysis Steps 34 Accuracy 35 Other Annex A Calibration Methods for Standard Concentrations of AFT B1, AFT B2, AFT G1 and AFT G Annex B Verification Methods for Immunoaffinity Column Annex C Tandem Mass Spectrometry Annex D Liquid Chromatogram Annex E Judgment Method for the Quality of Enzyme Linked Immunosorbent Assay Kit