Standard No.:	DB21/T 2469-2015
English Name:	Method of fluorescent RT-PCR for the detection of H1N1 swine influenza virus
Chinese Name:	H1N1亚型猪流感病毒荧光RT-PCR检测方法
Chinese Classification:	B41    Animal quarantine, veterinary and epidemic prevention
Professional Classification:	DB    Provincial Standard
Issued by:	Liaoning Provnicial Quality and Technology Supervision Bureau
Issued on:	2015-04-30
Implemented on:	2015-6-30
Status:	valid
Language:	English
File Format:	PDF

本标准规定了H1N1亚型猪流感病毒（swine influenza virus，SIV）荧光RT-PCR检测方法的范围、器材和试剂、引物和探针、操作步骤、结果判定和废弃物处理。
本标准适用于H1N1亚型猪流感的诊断、监测以及流行病学调查，适用于猪鼻咽棉拭子、肺脏及肺脏淋巴结、鸡胚尿囊培养液以及细胞培养物中H1N1亚型SIV HA和NA基因的检测。
本标准中的试验操作应在生物安全Ⅱ级（BSL-2）以上的实验室进行。