National Health Commission and State Administration for Market Regulation
Issued on:
2020-09-11
Implemented on:
2021-3-11
Status:
valid
Language:
English
File Format:
PDF
Word Count:
7500 words
Price(USD):
200.00
Delivery:
via email in 1 business day
GB 4789.44 -2020 National food safety standard - Food microbiological examination - Vibrio vulnificus
1 Scope
This standard specifies the examination method for Vibrio vulnificus in aquatic products.
This standard is applicable to the examination of Vibrio vulnificus in aquatic products such as fish, shrimp, crabs and shellfish.
2 Instruments and materials
In addition to the conventional sterilization and culture instruments in microbiological laboratory, other instruments and materials required are as follows:
2.1 Thermostatic incubator: 36°C±1°C.
2.2 Refrigerator: 2–5°C, 7–10°C.
2.3 Thermostatic water bath.
2.4 Homogenizer or sterile mortar.
2.5 Balance: With a sensitivity of 0.1 g.
2.6 PCR instrument.
2.7 Electrophoresis apparatus or capillary electrophoresis apparatus.
2.8 Gel electrophoresis imaging system or ultraviolet detector.
2.9 Biological safety cabinet.
2.10 High speed centrifuge (with maximum rotation speed greater than 15,000 r/min).
2.11 Vortex oscillator.
2.12 Micro-adjustable pipettes (measuring range 2.5 μL, 10 μL, 100 μL, and 1,000 μL) and matching tips.
2.13 Short-range pH paper or pH meter.
2.14 Sterile test tubes: 18 mm×180 mm and 15 mm×100 mm.
2.15 Sterile pipettes: 1 mL (with a scale division of 0.01 mL) and 10 mL (with a scale division of 0.1 mL).
2.16 Sterile conical flask: With a volume of 250 mL, 500 mL, or 1,000 mL.
2.17 Sterile culture dish: with a diameter of 90 mm.
1 Scope 2 Instruments and materials 3 Culture media and reagents 4 Examination procedures 5 Operation steps 6 Results and report Annex A Media and reagents
Standard
GB 4789.44-2020 National food safety standard - Food microbiological examination - Vibrio vulnificus (English Version)
Standard No.
GB 4789.44-2020
Status
valid
Language
English
File Format
PDF
Word Count
7500 words
Price(USD)
200.0
Implemented on
2021-3-11
Delivery
via email in 1 business day
Detail of GB 4789.44-2020
Standard No.
GB 4789.44-2020
English Name
National food safety standard - Food microbiological examination - Vibrio vulnificus
Chinese Name
食品安全国家标准 食品微生物学检验 创伤弧菌检验
Chinese Classification
X09
Professional Classification
GB
ICS Classification
Issued by
National Health Commission and State Administration for Market Regulation
GB 4789.44 -2020 National food safety standard - Food microbiological examination - Vibrio vulnificus
1 Scope
This standard specifies the examination method for Vibrio vulnificus in aquatic products.
This standard is applicable to the examination of Vibrio vulnificus in aquatic products such as fish, shrimp, crabs and shellfish.
2 Instruments and materials
In addition to the conventional sterilization and culture instruments in microbiological laboratory, other instruments and materials required are as follows:
2.1 Thermostatic incubator: 36°C±1°C.
2.2 Refrigerator: 2–5°C, 7–10°C.
2.3 Thermostatic water bath.
2.4 Homogenizer or sterile mortar.
2.5 Balance: With a sensitivity of 0.1 g.
2.6 PCR instrument.
2.7 Electrophoresis apparatus or capillary electrophoresis apparatus.
2.8 Gel electrophoresis imaging system or ultraviolet detector.
2.9 Biological safety cabinet.
2.10 High speed centrifuge (with maximum rotation speed greater than 15,000 r/min).
2.11 Vortex oscillator.
2.12 Micro-adjustable pipettes (measuring range 2.5 μL, 10 μL, 100 μL, and 1,000 μL) and matching tips.
2.13 Short-range pH paper or pH meter.
2.14 Sterile test tubes: 18 mm×180 mm and 15 mm×100 mm.
2.15 Sterile pipettes: 1 mL (with a scale division of 0.01 mL) and 10 mL (with a scale division of 0.1 mL).
2.16 Sterile conical flask: With a volume of 250 mL, 500 mL, or 1,000 mL.
2.17 Sterile culture dish: with a diameter of 90 mm.
Contents of GB 4789.44-2020
1 Scope
2 Instruments and materials
3 Culture media and reagents
4 Examination procedures
5 Operation steps
6 Results and report
Annex A Media and reagents
