GB/T 10649-2025 Determination of mixing homogeneity for trace mineral premix English, Anglais, Englisch, Inglés, えいご
This is a draft translation for reference among interesting stakeholders. The finalized translation (passing through draft translation, self-check, revision and verification) will be delivered upon being ordered.
ICS 65.120 CCS B 46
NATIONAL STANDARD OF THE PEOPLE'S REPUBLIC OF CHINA
GB/T 10649-2025 Replaces GB/T 10649-2008
Determination of Mixing Homogeneity for Trace Mineral Premix
Issued on 2025-08-29 | Implemented on 2026-03-01
Issued by
State Administration for Market Regulation
Standardization Administration of China
Contents
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Colorimetric method
5 Atomic absorption spectrometry
References
Determination of Mixing Homogeneity for Trace Mineral Premix
1 Scope
This document describes the colorimetric method and atomic absorption spectrometry method for the determination of mixing homogeneity of trace mineral premixes.
This document is applicable to the determination of mixing homogeneity of trace mineral premixes. Specifically, the colorimetric method applies to the determination of mixing homogeneity of trace mineral premixes supplemented with iron; atomic absorption spectrometry applies to the determination of mixing homogeneity of trace mineral premixes supplemented with copper.
2 Normative references
The following documents contain provisions which, through reference in this text, constitute provisions of this document. For dated references, only the editions cited apply. For undated references, the latest edition (including any amendments) applies.
GB/T 6682 Water for analytical laboratory use—Specification and test methods
GB/T 13885 Determination of the contents of calcium, copper, iron, magnesium, manganese, potassium, sodium, and zinc in feeds—Atomic absorption spectrometry
GB/T 20195 Animal feeding stuffs—Preparation of test samples
3 Terms and definitions
This document does not contain any terms and definitions that need to be defined.
4 Colorimetric method
4.1 Principle
After the sample is dissolved in acid, ferric ions are reduced to ferrous ions by hydroxylamine hydrochloride. The ferrous ions then react with the chromogenic agent 1,10-phenanthroline to form an orange-red complex. The absorbance of iron is measured colorimetrically. The mixing homogeneity of the product is evaluated using the coefficient of variation of absorbance values per unit mass from the same batch of samples.
4.2 Reagents or materials
Unless otherwise specified, use analytical grade reagents only.
4.2.1 Water: GB/T 6682, grade 3.
4.2.2 Hydrochloric acid.
4.2.3 Hydroxylamine hydrochloride solution (100 g/L): Weigh 10 g of hydroxylamine hydrochloride, dissolve it in water, dilute to 100 mL, mix well, transfer to a brown bottle, and store at 2 °C–8 °C.
4.2.4 Acetate buffer solution: Weigh 8.3 g of anhydrous sodium acetate, dissolve it in water, add 12 mL of glacial acetic acid, and dilute with water to 100 mL. Mix well.
4.2.5 1,10-Phenanthroline solution (1 g/L): Weigh 0.1 g of 1,10-phenanthroline, add 80 mL of water at 80°C to dissolve, cool, dilute to 100 mL, mix well, transfer to a brown bottle, and store at 2 °C–8 °C.
4.3 Instrumentation
4.3.1 Analytical balance: readability 0.0001 g.
4.3.2 Spectrophotometer: with 1 cm cuvettes.
4.4 Sample
4.4.1 Sampling
4.4.1.1 Samples required for this method should be collected separately.
4.4.1.2 Sampling at the production site:** Usually sample at the packing outlet. Select a specific production batch as the test batch. Based on the duration of this batch's production process, calculate the interval time for each sample according to equal time intervals. After the equipment runs stably, collect 10 samples randomly either manually or mechanically, with the mass of each sample being 50 g–100 g. No agitation or mixing should occur during sampling.
Note: If assessing the mixing performance of the mixer, sample at the mixer discharge outlet.
4.4.1.3 Sampling after packaging: Select a specific packaging batch as the test batch. Based on the total number of packages in this batch, randomly select 10 packages to obtain 10 samples, with the mass of each sample being 50 g–100 g. When sampling, each sample should be concentrated from a single point without any agitation or mixing.
4.4.2 Test portion preparation
Carry out according to the specifications of GB/T 20195.
4.5 Test procedure
Accurately weigh 1 g–10 g of the test portion (accurate to 0.0001 g) into a 250 mL beaker. Add a small amount of water to moisten, slowly add 20 mL of hydrochloric acid (4.2.2), mix well, add 50 mL of water, stir evenly, transfer to a 250 mL volumetric flask, make up to volume with water, mix well, and filter. Further dilute based on the iron content of the test portion, so that the mass concentration of iron in the test portion solution is 15 μg/mL. Precisely transfer 2 mL of the solution into a 25 mL volumetric flask, add 1 mL of hydroxylamine hydrochloride solution (4.2.3), mix thoroughly. After 5 minutes, add 5 mL of acetate buffer solution (4.2.4), mix well, then add 5 mL of 1,10-phenanthroline solution (4.2.5), dilute to 25 mL with water, mix thoroughly, let stand for 30 minutes. Using a reagent blank as reference, measure the absorbance of the test solution at a wavelength of 510 nm using the spectrophotometer.
Following this procedure, sequentially determine the absorbance values A₁, A₂, A₃, A₄ … A₁₀ for the 10 test portions from the same batch.
If the absorbance value exceeds the range of 0.2–0.8, repeat the test.
Standard
GB/T 10649-2025 Determination of mixing homogeneity for trace mineral premix (English Version)
Standard No.
GB/T 10649-2025
Status
to be valid
Language
English
File Format
PDF
Word Count
7000 words
Price(USD)
210.0
Implemented on
2026-3-1
Delivery
via email in 1~3 business day
Detail of GB/T 10649-2025
Standard No.
GB/T 10649-2025
English Name
Determination of mixing homogeneity for trace mineral premix
GB/T 10649-2025 Determination of mixing homogeneity for trace mineral premix English, Anglais, Englisch, Inglés, えいご
This is a draft translation for reference among interesting stakeholders. The finalized translation (passing through draft translation, self-check, revision and verification) will be delivered upon being ordered.
ICS 65.120 CCS B 46
NATIONAL STANDARD OF THE PEOPLE'S REPUBLIC OF CHINA
GB/T 10649-2025 Replaces GB/T 10649-2008
Determination of Mixing Homogeneity for Trace Mineral Premix
Issued on 2025-08-29 | Implemented on 2026-03-01
Issued by
State Administration for Market Regulation
Standardization Administration of China
Contents
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Colorimetric method
5 Atomic absorption spectrometry
References
Determination of Mixing Homogeneity for Trace Mineral Premix
1 Scope
This document describes the colorimetric method and atomic absorption spectrometry method for the determination of mixing homogeneity of trace mineral premixes.
This document is applicable to the determination of mixing homogeneity of trace mineral premixes. Specifically, the colorimetric method applies to the determination of mixing homogeneity of trace mineral premixes supplemented with iron; atomic absorption spectrometry applies to the determination of mixing homogeneity of trace mineral premixes supplemented with copper.
2 Normative references
The following documents contain provisions which, through reference in this text, constitute provisions of this document. For dated references, only the editions cited apply. For undated references, the latest edition (including any amendments) applies.
GB/T 6682 Water for analytical laboratory use—Specification and test methods
GB/T 13885 Determination of the contents of calcium, copper, iron, magnesium, manganese, potassium, sodium, and zinc in feeds—Atomic absorption spectrometry
GB/T 20195 Animal feeding stuffs—Preparation of test samples
3 Terms and definitions
This document does not contain any terms and definitions that need to be defined.
4 Colorimetric method
4.1 Principle
After the sample is dissolved in acid, ferric ions are reduced to ferrous ions by hydroxylamine hydrochloride. The ferrous ions then react with the chromogenic agent 1,10-phenanthroline to form an orange-red complex. The absorbance of iron is measured colorimetrically. The mixing homogeneity of the product is evaluated using the coefficient of variation of absorbance values per unit mass from the same batch of samples.
4.2 Reagents or materials
Unless otherwise specified, use analytical grade reagents only.
4.2.1 Water: GB/T 6682, grade 3.
4.2.2 Hydrochloric acid.
4.2.3 Hydroxylamine hydrochloride solution (100 g/L): Weigh 10 g of hydroxylamine hydrochloride, dissolve it in water, dilute to 100 mL, mix well, transfer to a brown bottle, and store at 2 °C–8 °C.
4.2.4 Acetate buffer solution: Weigh 8.3 g of anhydrous sodium acetate, dissolve it in water, add 12 mL of glacial acetic acid, and dilute with water to 100 mL. Mix well.
4.2.5 1,10-Phenanthroline solution (1 g/L): Weigh 0.1 g of 1,10-phenanthroline, add 80 mL of water at 80°C to dissolve, cool, dilute to 100 mL, mix well, transfer to a brown bottle, and store at 2 °C–8 °C.
4.3 Instrumentation
4.3.1 Analytical balance: readability 0.0001 g.
4.3.2 Spectrophotometer: with 1 cm cuvettes.
4.4 Sample
4.4.1 Sampling
4.4.1.1 Samples required for this method should be collected separately.
4.4.1.2 Sampling at the production site:** Usually sample at the packing outlet. Select a specific production batch as the test batch. Based on the duration of this batch's production process, calculate the interval time for each sample according to equal time intervals. After the equipment runs stably, collect 10 samples randomly either manually or mechanically, with the mass of each sample being 50 g–100 g. No agitation or mixing should occur during sampling.
Note: If assessing the mixing performance of the mixer, sample at the mixer discharge outlet.
4.4.1.3 Sampling after packaging: Select a specific packaging batch as the test batch. Based on the total number of packages in this batch, randomly select 10 packages to obtain 10 samples, with the mass of each sample being 50 g–100 g. When sampling, each sample should be concentrated from a single point without any agitation or mixing.
4.4.2 Test portion preparation
Carry out according to the specifications of GB/T 20195.
4.5 Test procedure
Accurately weigh 1 g–10 g of the test portion (accurate to 0.0001 g) into a 250 mL beaker. Add a small amount of water to moisten, slowly add 20 mL of hydrochloric acid (4.2.2), mix well, add 50 mL of water, stir evenly, transfer to a 250 mL volumetric flask, make up to volume with water, mix well, and filter. Further dilute based on the iron content of the test portion, so that the mass concentration of iron in the test portion solution is 15 μg/mL. Precisely transfer 2 mL of the solution into a 25 mL volumetric flask, add 1 mL of hydroxylamine hydrochloride solution (4.2.3), mix thoroughly. After 5 minutes, add 5 mL of acetate buffer solution (4.2.4), mix well, then add 5 mL of 1,10-phenanthroline solution (4.2.5), dilute to 25 mL with water, mix thoroughly, let stand for 30 minutes. Using a reagent blank as reference, measure the absorbance of the test solution at a wavelength of 510 nm using the spectrophotometer.
Following this procedure, sequentially determine the absorbance values A₁, A₂, A₃, A₄ … A₁₀ for the 10 test portions from the same batch.
If the absorbance value exceeds the range of 0.2–0.8, repeat the test.
