This standard specifies the method for determination of vitamin B6 content in feeds by high-performance liquid chromatography.
This standard is applicable to the determination of vitamin B6 in compound premix and vitamin premix with vitamin B6 content greater than 30 mg/kg.
2 Normative References
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this part. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.
GB/T 6682 Water for Laboratory Use — Specifications and Test Methods
GB/T 14699.1 Animal Feeding Stuffs — Sampling
3 Principle
After ultrasonic extraction of the acid extract of vitamin B6 specimen, the test solution is injected into a high-performance liquid chromatography (HPLC) system for separation. Ultraviolet (or diode matrix detector) is used for detection. The content of vitamin B6 can be calculated by external standard method.
4 Reagent and solution
Unless otherwise specified, the reagents used shall be of analytical grade, the water used shall be distilled water, and the deionized water for chromatographic use shall meet the requirements of Grade 1 water specified in GB/T 6682.1.
Foreword II 1 Scope 2 Normative References 3 Principle 4 Reagent and solution 5 Apparatus 6 Preparation of Specimen 7 Analytical Procedure 8 Calculation of Results 9 Repeatability
Standard
GB/T 14702-2018 Determination of vitamin B6 in premix—High performance liquid chromatograph (English Version)
Standard No.
GB/T 14702-2018
Status
valid
Language
English
File Format
PDF
Word Count
5500 words
Price(USD)
130.0
Implemented on
2019-4-1
Delivery
via email in 1 business day
Detail of GB/T 14702-2018
Standard No.
GB/T 14702-2018
English Name
Determination of vitamin B6 in premix—High performance liquid chromatograph
1 Scope
This standard specifies the method for determination of vitamin B6 content in feeds by high-performance liquid chromatography.
This standard is applicable to the determination of vitamin B6 in compound premix and vitamin premix with vitamin B6 content greater than 30 mg/kg.
2 Normative References
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this part. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.
GB/T 6682 Water for Laboratory Use — Specifications and Test Methods
GB/T 14699.1 Animal Feeding Stuffs — Sampling
3 Principle
After ultrasonic extraction of the acid extract of vitamin B6 specimen, the test solution is injected into a high-performance liquid chromatography (HPLC) system for separation. Ultraviolet (or diode matrix detector) is used for detection. The content of vitamin B6 can be calculated by external standard method.
4 Reagent and solution
Unless otherwise specified, the reagents used shall be of analytical grade, the water used shall be distilled water, and the deionized water for chromatographic use shall meet the requirements of Grade 1 water specified in GB/T 6682.1.
Contents of GB/T 14702-2018
Foreword II
1 Scope
2 Normative References
3 Principle
4 Reagent and solution
5 Apparatus
6 Preparation of Specimen
7 Analytical Procedure
8 Calculation of Results
9 Repeatability
