Standard No.:	T/CNCIA 03002-2020
English Name:	Test method for determining the antiviral activity of coating
Chinese Name:	涂料（漆膜）抗病毒性能测试方法
Chinese Classification:	G50    Basic standard and general method for coating
Professional Classification:	T/    Social Organization Standard
Source Content Issued by:	China National Coatings Industry Association
Issued on:	2020-11-24
Implemented on:	2021-1-1
Status:	valid
Target Language:	English
File Format:	PDF
Word Count:	8500 words
Translation Price(USD):	255.0
Delivery:	via email in 1 business day

T/CNCIA 03002-2020 Test method for determining the antiviral activity of coating Warning: Users of this standard shall have practical work experience in standardized laboratories. This document does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health measures and to ensure compliance with any national regulatory conditions. The laboratory shall meet the requirements of GB 19489, the test shall be carried out in a biosafety laboratory of BSL-2 or above safety level, and the biosafety of the laboratory shall be ensured. Waste generated during the test shall be treated as biohazard waste to ensure the safety of operators. 1 Scope This document specifies the terms and definitions, test principle, reagents and materials, apparatus, test preparation, test procedures, antivirus durability test and test report of the test for determining the antiviral activity of coating. This document is applicable to the determination of the antiviral activity of coatings (including water-based coatings, solvent-based coatings, radiation curable coatings and powder coatings). 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. GB/T 3186 Paints, varnishes and raw materials for paints and varnishes - Sampling GB/T 6682-2008 Water for analytical laboratory use - Specification and test methods GB/T 9278 Temperatures and humidities for conditioning and testing of paint specimens GB 19258 Ultraviolet germicidal lamp GB 19489 Laboratories - General requirements for biosafety YY 0569 Class II biological safety cabinets 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 virus microorganisms or genetic units that have no cellular structure and can only replicate within host cells, typically composed of proteins and a type of nucleic acid (DNA or RNA) 3.2 antiviral state where the number of infectious virus on surfaces of sample is reduced via physical or chemical measures 3.3 antiviral activity difference in the logarithm of the infectivity titer of virus found on an antiviral-treated sample and an untreated sample after inoculation with and contact to virus 3.4 antiviral rate percentage reduction of the infectivity titer of virus found on an antiviral-treated sample and an untreated sample after inoculation with and contact to virus 3.5 infectivity titer of virus number of infectious viral particles present per unit volume of cytolytic product or solution 3.6 plaque limited area formed in a cell monolayer under semisolid medium by a single infectious viral particle 3.7 plaque forming units; PFU unit expressing the concentration of the infectious virus per unit volume 3.8 plaque assay assay to determine the infectivity titer of virus from PFU by using the series of dilution 3.9 50% tissue culture infective dose, TCID50 concentration of an infectious virus in a viral eluent or diluent that causes 50% cytopathy 3.10 antivirus durability antiviral performance after a certain period of ultraviolet light irradiation by simulating the light aging mode of the product during use 4 Test principle The virus is inoculated on the prepared sample, and after a specific contact period, the virus reduction rate is calculated by comparing the number of the surviving viruses in the sample with that in the control sample. Two methods (plaque assay as specified in 8.5.1; TCID50 method as specified in 8.5.2) may be selected to calculate infectious virus titers. Note: The laboratory may select the virus calculation method according to its own experimental conditions and experimental techniques. 5 Reagents and materials 5.1 Test virus The test viruses are shown in Table 1. Other viruses may also be selected for the test, and the corresponding virus host cells may be selected at the same time. The entire operation process shall meet the biosafety requirements of the laboratory.

Foreword i 1 Scope 2 Normative references 3 Terms and definitions 4 Test principle 5 Reagents and materials 6 Apparatus 7 Test preparation 8 Test procedure 9 Antivirus durability test 10 Test report Annex A (Informative) Composition of EMEM Bibliography