YC/T 254-2008 Cigarettes - Determination of major carbonyls in mainstream cigarette smoke - High performance liquid chromatographic method (English Version)
Cigarettes - Determination of major carbonyls in mainstream cigarette smoke -
High performance liquid chromatographic method
1 Scope
This standard specifies the determination of major carbonyls in mainstream cigarette smoke by high performance liquid chromatographic method.
This standard is applicable to the determination of main carbonyls in mainstream cigarette smoke.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this standard. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.
GB/T 5606.1 Cigarettes - Sampling
GB/T 6682 Water for analytical laboratory use - Specification and test methods (GB/T 6682-1992; neq ISO 3696: 1987)
GB/T 19609 Cigarette - Determination of total and nicotine-free dry particulate matter using a routine analytical smoking machine (GB/T 19609-2004; ISO 4387: 2000, MOD)
3 Principle
Carbonyls in mainstream cigarette smoke are captured and derivatized by fiberglass filter leaf treated with 2,4-dinitrophenylhydrazine (DNPH) solution, and the main carbonyls in mainstream cigarette smoke are quantitatively analyzed by quantitatively determining the derivative compounds with high performance liquid chromatography.
4 Reagents
4.1 Unless otherwise specified, reagents of analytically pure grade shall be used.
4.2 Deionized water, which shall comply with GB/T 6682, with R>5 MΩ (filtered by 0.45 μm filter membrane before use).
4.3 Acetonitrile, chromatographically pure (or that of analytically pure, used after being redistilled).
4.4 Tetrahydrofuran, chromatographically pure (or that of analytically pure, used after being redistilled).
4.5 Isopropanol, chromatographically pure (or that of analytically pure, used after being redistilled).
4.6 Perchloric acid.
Foreword i
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Analytical procedures
8 Result calculation
9 Precision, recovery rate and detection limit
10 Inspection report
Annex A (Informative) Chromatogram examples
Annex B (Informative) Experimental results of precision, recovery rate and detection limit of this method
Standard
YC/T 254-2008 Cigarettes - Determination of major carbonyls in mainstream cigarette smoke - High performance liquid chromatographic method (English Version)
Standard No.
YC/T 254-2008
Status
valid
Language
English
File Format
PDF
Word Count
5000 words
Price(USD)
150.0
Implemented on
2008-4-14
Delivery
via email in 1 business day
Detail of YC/T 254-2008
Standard No.
YC/T 254-2008
English Name
Cigarettes - Determination of major carbonyls in mainstream cigarette smoke - High performance liquid chromatographic method
Cigarettes - Determination of major carbonyls in mainstream cigarette smoke -
High performance liquid chromatographic method
1 Scope
This standard specifies the determination of major carbonyls in mainstream cigarette smoke by high performance liquid chromatographic method.
This standard is applicable to the determination of main carbonyls in mainstream cigarette smoke.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this standard. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.
GB/T 5606.1 Cigarettes - Sampling
GB/T 6682 Water for analytical laboratory use - Specification and test methods (GB/T 6682-1992; neq ISO 3696: 1987)
GB/T 19609 Cigarette - Determination of total and nicotine-free dry particulate matter using a routine analytical smoking machine (GB/T 19609-2004; ISO 4387: 2000, MOD)
3 Principle
Carbonyls in mainstream cigarette smoke are captured and derivatized by fiberglass filter leaf treated with 2,4-dinitrophenylhydrazine (DNPH) solution, and the main carbonyls in mainstream cigarette smoke are quantitatively analyzed by quantitatively determining the derivative compounds with high performance liquid chromatography.
4 Reagents
4.1 Unless otherwise specified, reagents of analytically pure grade shall be used.
4.2 Deionized water, which shall comply with GB/T 6682, with R>5 MΩ (filtered by 0.45 μm filter membrane before use).
4.3 Acetonitrile, chromatographically pure (or that of analytically pure, used after being redistilled).
4.4 Tetrahydrofuran, chromatographically pure (or that of analytically pure, used after being redistilled).
4.5 Isopropanol, chromatographically pure (or that of analytically pure, used after being redistilled).
4.6 Perchloric acid.
Contents of YC/T 254-2008
Foreword i
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Analytical procedures
8 Result calculation
9 Precision, recovery rate and detection limit
10 Inspection report
Annex A (Informative) Chromatogram examples
Annex B (Informative) Experimental results of precision, recovery rate and detection limit of this method
