Standard No.:	GB 7300.505-2025
English Name:	Feed additives—Part 5:Live Microorganisms—Bacillus coagulans
Chinese Name:	饲料添加剂 第5部分：微生物 凝结芽孢杆菌
Chinese Classification:	B46    Livestock and poultry feed and additive
Professional Classification:	GB    National Standard
ICS Classification:	65.12 65.12    Animal feeding stuffs 65.12
Source Content Issued by:	SAMR; SAC
Issurane Date:	2025-6-30
Implementation Date:	2026-7-1
Status:	valid
Target Language:	English
File Format:	PDF
Word Count:	8500 words
Translation Price(USD):	255.0
Delivery:	via email in 1 business day

GB 7300.505-2025 Feed additives - Part 5: Live Microorganisms - Bacillus coagulans 1 Scope This document specifies the raw materials and bacterial strains of feed additive Bacillus coagulans, as well as technical requirements, sampling, inspection rules, labeling, packaging, transport, storage, and shelf life, and describes the applicable test methods for these items. This document applies to feed additive Bacillus coagulans produced by fermentation with culture strain Bacillus coagulans, addition or non-addition of carriers, drying and other processes. 2 Normative references The contents in the following documents constitute essential provisions of this document through normative references in the text. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. GB/T 5917.1 Determination of feed particle size - Two-sieve screening method GB/T 6435 Determination of moisture in feedstuffs GB/T 8170 Rules of rounding off for numerical values & expression and judgment of limiting values GB 10648 Feed label GB/T 13079 Determination of total arsenic in feeds GB/T 13080 Determination of lead in feeds - Atomic absorption spectrometry GB/T 13081 Determination of mercury in feeds GB/T 13082 Determination of cadmium in feeds GB/T 13091 Determination of salmonella in feeds GB/T 13092 Enumeration of molds count in feeds GB/T 14699 Feeding stuffs - Sampling GB/T 18869-2019 Determination of coliform in feeds GB/T 42959 Microbiological examination of feed - Sampling NY/T 2071 Determination of aflatoxins, zearalenone and T-2 in feeds - Liquid chromatography-tandem mass spectrometry 3 Terms and Definitions For the purposes of this document, the following terms and definitions apply. 3.1 Bacillus coagulans Weizmannia coagulans Heyndrickxia coagulans one of genus Heyndrickxia, family Bacillaceae, phylum Firmicutes Note to the entry: Gram-positive; individual bacteria are oval or rod-shaped, non-flagellated, and spore-forming; spores are oval or spherical, located within the sporangium, central to subterminal, and motile. 4 Raw materials and bacterial strains 4.1 Raw materials They are derived from the varieties listed in Catalogue of Feed Ingredients and/or Catalogue of feed additive varieties. 4.2 Bacterial strain The bacterial strain is Bacillus coagulan. 5 Technical requirements 5.1 Appearance and properties The products have uniform color, no lumps, no mold and no strange odor. NOTE: If the product has a distinct fermented odor, it is deemed normal. 5.2 Identification of Bacillus coagulans The morphological characteristics, physiological and biochemical characteristics, and molecular biological results shall comply with Annex A. 5.3 Quality index It shall comply with Table 1. Table 1 Quality index

Contents Foreword i Introduction iv 1 Scope 2 Normative references 3 Terms and Definitions 4 Raw materials and bacterial strains 5 Technical requirements 6 Sampling 7 Test method 8 Inspection rules 9 Labeling, packaging, transport, storage, and shelf life 10 Implementation of the standard Annex A (Normative) Identification of Bacillus coagulans Annex B (Normative) Method for determination of viable count of Bacillus coagulans Bibliography