Standard No.:	GB 22255-2014
English Name:	National Food Safety Standard Determination of Sucralose in Foods
Chinese Name:	食品安全国家标准 食品中三氯蔗糖（蔗糖素）的测定
Chinese Classification:	X09    Hygiene, safety and labor protection
Professional Classification:	GB    National Standard
Source Content Issued by:	Health and Family Planning Commission
Issued on:	2015-01-28
Implemented on:	2015-7-28
Status:	superseded
Superseded by:	GB 5009.298-2023 National food safety standard - Determination of sucralose in foods
Superseded on:	2024-9-6
Superseding:	GB/T 22255-2008 Determination of sucralose in foods
Target Language:	English
File Format:	PDF
Word Count:	3000 words
Translation Price(USD):	60.0
Delivery:	via email in 1 business day

1 Scope This standard specifies the determination method of sucralose in foods. This standard is applicable to the determination of sucralose in foods. 2 Theory Extract the sucralose in the specimen with methanol aqueous solution, remove protein and fat, then separate it with high performance liquid chromatograph and reversed-phase C18 chromatographic column after purifying and gathering it with solid-phase extraction column, detect it with evaporative light-scattering detector or refractive index detector, conduct qualitative analysis according to retention time, and quantify it by peak area. 3 Reagents and Materials Note: Unless otherwise specified, analytically pure reagents and Grade 1 water (specified in GB/T 6682) are adopted for the purpose of this method. 3.1 Reagents 3.1.1 Methanol (CH3OH). 3.1.2 Acetonitrile (CH3CN): chromatographically pure. 3.1.3 N-hexane (C6H14). 3.1.4 Zinc acetate [Zn(CH3COO)2•2H2O].

Foreword i 1 Scope 2 Theory 3 Reagents and Materials 4 Apparatuses 5 Analytical Procedures 6 Expression of Analysis Results 7 Precision 8 Others Annex A The Elution Program of Liquid Chromatography under Complex Conditions (Applicable to Evaporative Light-scattering Detector) Annex B The High performance Liquid Chromatograms of Sucralose