1 Scope
This standard specifies the determination methods of Vitamins A, E and D in foods.
Method I of this standard is applicable to the determination of Vitamins A and E in foods.
Method II of this standard is applicable to the determination of Vitamins E in foods such as edible oil, nuts, beans and chili powder.
Method III of the standard is applicable to the determination of Vitamins D2 and D3 in foods.
Method IV of this standard is applicable to the determination of Vitamins D2 and D3 in formula foods.
Method I Determination of Vitamins A and E in Foods Reversed-phase High Performance Liquid Chromatography
2 Principle
After subjecting to saponification (subjecting to enzymolysis with amylase first for starch-containing specimen), extraction, purification and concentration, Vitamins A and E in the specimen are separated by C30 or PFP reversed-phase liquid chromatographic column, detected with ultraviolet detector or fluorescence detector and quantitated by external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Grade I water (defined in GB/T 6682) are adopted for the purposes of this method.
3.1 Reagents
3.1.1 Absolute ethanol (C2H5OH): without aldehyde substance after inspection, see A.1 for inspection method.
3.1.2 Ascorbic acid (C6H8O6).
3.1.3 Potassium hydroxide (KOH).
3.1.4 Ethyl ether [(CH3CH2)2O]: without peroxide after inspection, see A.2 for inspection method.
3.1.5 Petroleum ether (C5H12O2): with boiling range of 30℃~60℃.
3.1.6 Anhydrous sodium sulfate (Na2SO4).
3.1.7 pH test paper (at the pH range of 1~14).
3.1.8 Methanol (CH3OH): chromatographically pure.
3.1.9 Amylase: activity unit≥100U/mg.
3.1.10 2,6-Di-tert-butyl-4-methylphenol (C15H24O): referred to as BHT.
3.2 Reagent preparation
3.2.1 Potassium hydroxide solution (50g/100g): weigh 50g of potassium hydroxide, dissolve it with 50mL of water, after cooling, store it in a polyethylene bottle.
3.2.2 Petroleum ether - ethyl ether solution (1+1): measure 200mL of petroleum ether and add into 200mL of ethyl ether, mix them well.
Contents
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Apparatuses
5 Analysis Procedures
6 Expression of Analysis Results
7 Accuracy
8 Others
9 Principle
10 Reagents and Materials
11 Apparatuses
12 Analysis Procedures
13 Expression of Analysis Results
14 Accuracy
15 Others
16 Principle
17 Reagents and Materials
18 Apparatuses
19 Analysis Procedures
20 Expression of Analysis Results
21 Accuracy
22 Others
23 Principle
24 Reagents and Materials
25 Apparatuses
26 Analysis Procedures
27 Expression of Analysis Results
28 Accuracy
29 Others
Appendix A
Appendix B Calibration Method for Concentrations of Vitamins A, D and E Standard Solutions
Appendix C Chromatogram
Standard
GB 5009.82-2016 National Food Safety Standard -- Determination of Vitamins A, D and E in Foods (English Version)
Standard No.
GB 5009.82-2016
Status
valid
Language
English
File Format
PDF
Word Count
11000 words
Price(USD)
130.0
Implemented on
2017-6-23
Delivery
via email in 1 business day
Detail of GB 5009.82-2016
Standard No.
GB 5009.82-2016
English Name
National Food Safety Standard -- Determination of Vitamins A, D and E in Foods
Chinese Name
食品安全国家标准 食品中维生素A、D、E的测定
Chinese Classification
Professional Classification
GB
ICS Classification
Issued by
National Health and Family Planning Commission; China Food and Drug Administration
1 Scope
This standard specifies the determination methods of Vitamins A, E and D in foods.
Method I of this standard is applicable to the determination of Vitamins A and E in foods.
Method II of this standard is applicable to the determination of Vitamins E in foods such as edible oil, nuts, beans and chili powder.
Method III of the standard is applicable to the determination of Vitamins D2 and D3 in foods.
Method IV of this standard is applicable to the determination of Vitamins D2 and D3 in formula foods.
Method I Determination of Vitamins A and E in Foods Reversed-phase High Performance Liquid Chromatography
2 Principle
After subjecting to saponification (subjecting to enzymolysis with amylase first for starch-containing specimen), extraction, purification and concentration, Vitamins A and E in the specimen are separated by C30 or PFP reversed-phase liquid chromatographic column, detected with ultraviolet detector or fluorescence detector and quantitated by external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Grade I water (defined in GB/T 6682) are adopted for the purposes of this method.
3.1 Reagents
3.1.1 Absolute ethanol (C2H5OH): without aldehyde substance after inspection, see A.1 for inspection method.
3.1.2 Ascorbic acid (C6H8O6).
3.1.3 Potassium hydroxide (KOH).
3.1.4 Ethyl ether [(CH3CH2)2O]: without peroxide after inspection, see A.2 for inspection method.
3.1.5 Petroleum ether (C5H12O2): with boiling range of 30℃~60℃.
3.1.6 Anhydrous sodium sulfate (Na2SO4).
3.1.7 pH test paper (at the pH range of 1~14).
3.1.8 Methanol (CH3OH): chromatographically pure.
3.1.9 Amylase: activity unit≥100U/mg.
3.1.10 2,6-Di-tert-butyl-4-methylphenol (C15H24O): referred to as BHT.
3.2 Reagent preparation
3.2.1 Potassium hydroxide solution (50g/100g): weigh 50g of potassium hydroxide, dissolve it with 50mL of water, after cooling, store it in a polyethylene bottle.
3.2.2 Petroleum ether - ethyl ether solution (1+1): measure 200mL of petroleum ether and add into 200mL of ethyl ether, mix them well.
Contents of GB 5009.82-2016
Contents
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Apparatuses
5 Analysis Procedures
6 Expression of Analysis Results
7 Accuracy
8 Others
9 Principle
10 Reagents and Materials
11 Apparatuses
12 Analysis Procedures
13 Expression of Analysis Results
14 Accuracy
15 Others
16 Principle
17 Reagents and Materials
18 Apparatuses
19 Analysis Procedures
20 Expression of Analysis Results
21 Accuracy
22 Others
23 Principle
24 Reagents and Materials
25 Apparatuses
26 Analysis Procedures
27 Expression of Analysis Results
28 Accuracy
29 Others
Appendix A
Appendix B Calibration Method for Concentrations of Vitamins A, D and E Standard Solutions
Appendix C Chromatogram
