Standard No.:	GB/T 5009.82-2003
English Name:	Determination of retinol and tocopherol in foods
Chinese Name:	食品中维生素A和维生素E的测定
Chinese Classification:	C53    Food sanitation
Professional Classification:	GB    National Standard
Source Content Issued by:	MOH; SAC
Issued on:	2003-8-11
Implemented on:	2004-1-1
Status:	superseded
Superseded by:	GB 5009.82-2016 National Food Safety Standard -- Determination of Vitamins A, D and E in Foods
Superseded on:	2017-6-23
Superseding:	GB/T 12388-1990 Method for determination of retinol and tocopherol in foods
Target Language:	English
File Format:	PDF
Word Count:	3000 words
Translation Price(USD):	60.0
Delivery:	via email in 1 business day

1 Scope This standard specifies the method for the determination of retinol and tocopherol in foods. This standard is applicable to the determination of retinol and tocopherol in foods. The detection limits of this standard are: VA: 0.8ng; α-E: 91.8ng; γ-E: 36.6ng; δ-E: 20.6ng. Method I High Performance Liquid Chromatography 2 Principle After saponification treatment, retinol and tocopherol in the specimen shall be extracted from the non-saponifiable part into the organic solvent, separated with high performance liquid chromatography C18 reversed phase column, detected by UV detector and then determined quantitatively with internal standard method.

Contents Foreword I 1 Scope Method I High Performance Liquid Chromatography 2 Principle 3 Reagents 4 Instruments and Equipment 5 Analysis Procedures 6 Result Calculation 7 Precision Method 2: Colorimetric Method 8 Principle 9 Reagents 10 Instruments 11 Analysis Procedures 12 Determination 13 Result calculation 14 Precision