1 Scope
This standard specifies methods of preparing the methyl esters of fatty acids.
This standard includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, three methylation methods are specified, as follows:
a) Boron trifluoride (BF3) method (see Clause 3);
b) Trimethylsulfonium hydroxide (TMSH) method (see Clause 4);
c) Trans-esterification method (see Clause 5).
Methyl esters so produced are used in various analytical procedures requiring such derivatives, for example gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).
2 Normative References
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.
GB 6682 Water for Analytical Laboratory Use — Specification and Test Methods (GB 6682-2008, ISO 3696:2008, MOD)
GB/T 15687 Animal and Vegetable Fats and Oils — Preparation of Test Sample (GB/T 15687-2008, ISO 661:2003, IDT)
3 General Method Using Boron Trifluoride
1 Scope
This standard specifies methods of preparing the methyl esters of fatty acids.
This standard includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, three methylation methods are specified, as follows:
a) Boron trifluoride (BF3) method (see Clause 3);
b) Trimethylsulfonium hydroxide (TMSH) method (see Clause 4);
c) Trans-esterification method (see Clause 5).
Methyl esters so produced are used in various analytical procedures requiring such derivatives, for example gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).
2 Normative References
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.
GB 6682 Water for Analytical Laboratory Use — Specification and Test Methods (GB 6682-2008, ISO 3696:2008, MOD)
GB/T 15687 Animal and Vegetable Fats and Oils — Preparation of Test Sample (GB/T 15687-2008, ISO 661:2003, IDT)
3 General Method Using Boron Trifluoride
