Standard No.:	GB/T 5009.197-2003
English Name:	Determination of thiamine hydrochloride, pyridoxine hydrochloride, niacin, niacinamide and caffeine in health foods
Chinese Name:	保健食品中盐酸硫胺素、盐酸吡哆醇、烟酸、烟酰胺和咖啡因的测定
Chinese Classification:	C53    Food sanitation
Professional Classification:	GB    National Standard
Source Content Issued by:	AQSIQ
Issued on:	2003-8-11
Implemented on:	2004-1-1
Status:	superseded
Superseded by:	GB/T 45243-2025 Determination of thiamine, riboflavin , pyridoxine, niacin, niacinamide and caffeine in health foods
Superseded on:	2025-8-1
Abolished on:	2025-08-01
Target Language:	English
File Format:	PDF
Word Count:	2000 words
Translation Price(USD):	40.0
Delivery:	via email in 1 business day

1 Scope This standard specifies the high performance liquid chromatography determination method of thiamine hydrochloride, pyridoxine hydrochloride, niacin, niacinamide and caffeine in health foods. This standard is applicable to the high performance liquid chromatography determination method for adding thiamine hydrochloride, pyridoxine hydrochloride, niacin, niacinamide and caffeine serving as dietary supplements into test sample types such as tablet, capsule, oral liquid and drink. Detection limit of this method is: 0.02μg for thiamine hydrochloride, 0.02μg for pyridoxine hydrochloride, 0.10μg for niacin, 0.06μg for niacinamide and 0.05μg for caffeine. The linear range is: 0.500μg/mL~50.0μg/mL for thiamine hydrochloride; 0.500μg/mL~50.0μg/mL for pyridoxine hydrochloride; 1.00μg/mL~100μg/mL for niacin; 1.00μg/mL~100μg/mL for niacinamide; and 5.00μg/mL~500μg/mL for caffeine.

Contents Foreword Introduction 1 Scope 2 Principle 3 Reagents 4 Instruments 5 Analysis Procedures 6 Result Calculation 7 Precision