Standard No.:	GB/T 5009.86-2003
English Name:	Determination of total ascorbic acid in fruits, vegetables and derived products-Fluorometric method
Chinese Name:	蔬菜、水果及其制品中总抗坏血酸的测定(荧光法和2，4？二硝基苯肼法)
Chinese Classification:	C53    Food sanitation
Professional Classification:	GB    National Standard
Issued by:	AQSIQ
Issued on:	2003-8-11
Implemented on:	2004-1-1
Status:	superseded
Superseded by:	GB 5009.86-2016 National Food Safety Standard-Determination of Ascorbic Acid in Foods
Superseded on:	2017-3-1
Abolished on:	2017-03-01
Superseding:	GB/T 12392-1990 Method for determination of total ascorbic acid in fruits, vegetables and derived products―Fluorometric method and colorimetric method 2.4-dinitrophenylhydrazine
Language:	English
File Format:	PDF
Word Count:	3000 words
Price(USD):	224.00
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1 Scope



This standard specifies determination of total ascorbic acid in the food in fluorometric method and colorimetric method.

This standard is applicable to determination of total ascorbic acid in fruits, vegetables and derived products

Detection limit of this method: 0.022μg/mL for fluorometric method and 0.1μg/mL for colorimetric method. Linear range: 5~20μg/mL for fluorometric method and 1~12μg/mL for colorimetric method.

Contents
Foreword I
1 Scope
2 Principle
3 Reagents
4 Instruments
5 Analysis Procedures
6 Result Calculation
7 Precision
8 Principle
9 Reagents
10 Instruments
11 Analysis Procedures
12 Result Calculation
13 Precision