1 Scope
This standard specifies determination method of tartaric acid, lactic acid, malic acid, citric acid, succinic acid, fumaric acid and adipic acid in foods.
This standard is applicable to seven organic acids in fruit juice and fruit juice beverage, carbonated beverage, gum candy, biscuit, pastry, jelly, fruit can, dough product and baked food filling.
2 Principle
Directly dilute or extract specimen with water, purify with strong anion exchanging solid phase extraction column, separate with reversed-phase chromatographic column and conduct qualitative determination by retention time and quantitative determination by external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Class-I water (defined in GB/T 6682) are adopted for the purpose of this method.
3.1 Reagents
3.1.1 Methanol (CH3OH): chromatographically pure.
3.1.2 Absolute ethanol (CH3CH2OH): chromatographically pure.
3.1.3 Phosphoric acid (H3PO4).
3.2 Preparation of reagents
3.2.1 Phosphoric acid solution (0.1%): measure 2 mL phosphoric acid, add water to 100 mL and mix well.
3.2.2 Phosphoric acid-methanol solution (2%): measure 2 mL phosphoric acid, add water to 100 mL and mix well.
3.3 Standard products
3.3.1 Lactic acid (C3H6O3): purity≥99%.
3.3.2 Tartaric acid (C4H6O6): purity≥99%.
3.3.3 Malic acid (C4H6O5): purity≥99%.
3.3.4 Citric acid (C6H8N7): purity ≥98%.
3.3.5 Tartaric acid (C4H6N4): purity≥99%.
3.3.6 Fumaric acid (C4H4O4): purity≥99%.
3.3.7 Adipic acid (C6H10N4): purity≥99%.
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Procedures
6 Expression of Analysis Results
7 Accuracy
8 Other
Annex A Standard Chromatogram of Acidity Regulator
Standard
GB 5009.157-2016 National Food Safety Standard - Determination of organic acids in food (English Version)
Standard No.
GB 5009.157-2016
Status
valid
Language
English
File Format
PDF
Word Count
2500 words
Price(USD)
70.0
Implemented on
2017-3-1
Delivery
via email in 1 business day
Detail of GB 5009.157-2016
Standard No.
GB 5009.157-2016
English Name
National Food Safety Standard - Determination of organic acids in food
1 Scope
This standard specifies determination method of tartaric acid, lactic acid, malic acid, citric acid, succinic acid, fumaric acid and adipic acid in foods.
This standard is applicable to seven organic acids in fruit juice and fruit juice beverage, carbonated beverage, gum candy, biscuit, pastry, jelly, fruit can, dough product and baked food filling.
2 Principle
Directly dilute or extract specimen with water, purify with strong anion exchanging solid phase extraction column, separate with reversed-phase chromatographic column and conduct qualitative determination by retention time and quantitative determination by external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Class-I water (defined in GB/T 6682) are adopted for the purpose of this method.
3.1 Reagents
3.1.1 Methanol (CH3OH): chromatographically pure.
3.1.2 Absolute ethanol (CH3CH2OH): chromatographically pure.
3.1.3 Phosphoric acid (H3PO4).
3.2 Preparation of reagents
3.2.1 Phosphoric acid solution (0.1%): measure 2 mL phosphoric acid, add water to 100 mL and mix well.
3.2.2 Phosphoric acid-methanol solution (2%): measure 2 mL phosphoric acid, add water to 100 mL and mix well.
3.3 Standard products
3.3.1 Lactic acid (C3H6O3): purity≥99%.
3.3.2 Tartaric acid (C4H6O6): purity≥99%.
3.3.3 Malic acid (C4H6O5): purity≥99%.
3.3.4 Citric acid (C6H8N7): purity ≥98%.
3.3.5 Tartaric acid (C4H6N4): purity≥99%.
3.3.6 Fumaric acid (C4H4O4): purity≥99%.
3.3.7 Adipic acid (C6H10N4): purity≥99%.
Contents of GB 5009.157-2016
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Procedures
6 Expression of Analysis Results
7 Accuracy
8 Other
Annex A Standard Chromatogram of Acidity Regulator