1 Scope
This standard specifies the method for determining taurine in foods.
This standard is applicable to determination of taurine in formula foods for infant and young children, milk powder, soya flour, soybean milk, milk beverage, beverages for special uses, flavored drinks, solid beverage and jelly.
Method I O-Phthalaldehyde (OPA) Post-column Derivatization High Performance Liquid Chromatography
2 Principle
Dissolve the specimen with water, precipitate protein with metaphosphoric acid, extract through ultrasonic vibration, centrifuge and filter with microporous membrane, separate with sodium ion chromatographic column, carry out derivation reaction with o-Phthalaldehyde (OPA), detect with fluorescence detector and quantify by using external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Grade-I water (defined in GB/T 6682) are adopted for the purposes of this method.
3.1 Reagents
3.1.1 Metaphosphoric acid.
Contents
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Steps
6 Expression of Analysis Results
7 Precision
8 Others
9 Principle
10 Reagents and Materials
11 Instruments and Apparatus
12 Analysis Steps
13 Expression of Analysis Results
14 Precision
15 Others
Appendix A Chromatogram
Standard
GB 5009.169-2016 National Food Safety Standard Determination of Taurine in Foods (English Version)
Standard No.
GB 5009.169-2016
Status
valid
Language
English
File Format
PDF
Word Count
5500 words
Price(USD)
80.0
Implemented on
2017-3-1
Delivery
via email in 1 business day
Detail of GB 5009.169-2016
Standard No.
GB 5009.169-2016
English Name
National Food Safety Standard Determination of Taurine in Foods
1 Scope
This standard specifies the method for determining taurine in foods.
This standard is applicable to determination of taurine in formula foods for infant and young children, milk powder, soya flour, soybean milk, milk beverage, beverages for special uses, flavored drinks, solid beverage and jelly.
Method I O-Phthalaldehyde (OPA) Post-column Derivatization High Performance Liquid Chromatography
2 Principle
Dissolve the specimen with water, precipitate protein with metaphosphoric acid, extract through ultrasonic vibration, centrifuge and filter with microporous membrane, separate with sodium ion chromatographic column, carry out derivation reaction with o-Phthalaldehyde (OPA), detect with fluorescence detector and quantify by using external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Grade-I water (defined in GB/T 6682) are adopted for the purposes of this method.
3.1 Reagents
3.1.1 Metaphosphoric acid.
Contents of GB 5009.169-2016
Contents
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Steps
6 Expression of Analysis Results
7 Precision
8 Others
9 Principle
10 Reagents and Materials
11 Instruments and Apparatus
12 Analysis Steps
13 Expression of Analysis Results
14 Precision
15 Others
Appendix A Chromatogram