1 Scope
This standard specifies the methods of determining pantothenic acid and calcium pantothenate in foods.
Method I in this standard is applicable to determining pantothenic acid in foods, and Method II is applicable to determining pantothenic acid in health foods and formula foods of nutrient supplement category.
Method I Microbiological Method
2 Principle
Pantothenic acid is a necessary nutrient for growth of Lactobacillus plantarum (ATCC 8014). Under certain control condition, inoculate Lactobacillus plantarum in culture solution containing sample solution, after a certain period, determine light transmittance (or absorbance), then calculate the content of pantothenic acid in the sample according to the standard curve of pantothenic acid content and light transmittance (or absorbance).
3 Reagents and Materials
Unless otherwise specified, all reagents adopted for this method are analytically pure and water is Grade II water as specified in GB/T 6682.
3.1 Reagents
3.1.1 Hydrochloric acid (HCl).
3.1.2 Glacial acetic acid (C2H4O2).
3.1.3 Sodium hydroxide (NaOH).
3.1.4 Sodium chloride (NaCl).
3.1.5 Sodium carbonate (Na2CO3).
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Equipment
5 Preparation and Preservation of Strain
6 Analysis Procedures
7 Accuracy
8 Other
9 Principle
10 Reagents and Materials
11 Instruments and Equipment
12 Analysis Procedures
13 Expression of Analysis Results
14 Accuracy
15 Other
Annex A Preparation Method of Culture Solution for Pantothenic Acid Determination
Annex B Liquid Chromatogram of Standard Pantothenic Acid Solution
Standard
GB 5009.210-2016 National Food Safety Standard - Determination of pantothenic acid in food (English Version)
Standard No.
GB 5009.210-2016
Status
superseded
Language
English
File Format
PDF
Word Count
7000 words
Price(USD)
130.0
Implemented on
2017-3-1
Delivery
via email in 1 business day
Detail of GB 5009.210-2016
Standard No.
GB 5009.210-2016
English Name
National Food Safety Standard - Determination of pantothenic acid in food
1 Scope
This standard specifies the methods of determining pantothenic acid and calcium pantothenate in foods.
Method I in this standard is applicable to determining pantothenic acid in foods, and Method II is applicable to determining pantothenic acid in health foods and formula foods of nutrient supplement category.
Method I Microbiological Method
2 Principle
Pantothenic acid is a necessary nutrient for growth of Lactobacillus plantarum (ATCC 8014). Under certain control condition, inoculate Lactobacillus plantarum in culture solution containing sample solution, after a certain period, determine light transmittance (or absorbance), then calculate the content of pantothenic acid in the sample according to the standard curve of pantothenic acid content and light transmittance (or absorbance).
3 Reagents and Materials
Unless otherwise specified, all reagents adopted for this method are analytically pure and water is Grade II water as specified in GB/T 6682.
3.1 Reagents
3.1.1 Hydrochloric acid (HCl).
3.1.2 Glacial acetic acid (C2H4O2).
3.1.3 Sodium hydroxide (NaOH).
3.1.4 Sodium chloride (NaCl).
3.1.5 Sodium carbonate (Na2CO3).
Contents of GB 5009.210-2016
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Equipment
5 Preparation and Preservation of Strain
6 Analysis Procedures
7 Accuracy
8 Other
9 Principle
10 Reagents and Materials
11 Instruments and Equipment
12 Analysis Procedures
13 Expression of Analysis Results
14 Accuracy
15 Other
Annex A Preparation Method of Culture Solution for Pantothenic Acid Determination
Annex B Liquid Chromatogram of Standard Pantothenic Acid Solution