1 Scope
This standard specifies the determination method of sucralose in foods.
This standard is applicable to the determination of sucralose in foods.
2 Theory
Extract the sucralose in the specimen with methanol aqueous solution, remove protein and fat, then separate it with high performance liquid chromatograph and reversed-phase C18 chromatographic column after purifying and gathering it with solid-phase extraction column, detect it with evaporative light-scattering detector or refractive index detector, conduct qualitative analysis according to retention time, and quantify it by peak area.
3 Reagents and Materials
Note: Unless otherwise specified, analytically pure reagents and Grade 1 water (specified in GB/T 6682) are adopted for the purpose of this method.
3.1 Reagents
3.1.1 Methanol (CH3OH).
3.1.2 Acetonitrile (CH3CN): chromatographically pure.
3.1.3 N-hexane (C6H14).
3.1.4 Zinc acetate [Zn(CH3COO)2•2H2O].
Foreword i
1 Scope
2 Theory
3 Reagents and Materials
4 Apparatuses
5 Analytical Procedures
6 Expression of Analysis Results
7 Precision
8 Others
Annex A The Elution Program of Liquid Chromatography under Complex Conditions (Applicable to Evaporative Light-scattering Detector)
Annex B The High performance Liquid Chromatograms of Sucralose
1 Scope
This standard specifies the determination method of sucralose in foods.
This standard is applicable to the determination of sucralose in foods.
2 Theory
Extract the sucralose in the specimen with methanol aqueous solution, remove protein and fat, then separate it with high performance liquid chromatograph and reversed-phase C18 chromatographic column after purifying and gathering it with solid-phase extraction column, detect it with evaporative light-scattering detector or refractive index detector, conduct qualitative analysis according to retention time, and quantify it by peak area.
3 Reagents and Materials
Note: Unless otherwise specified, analytically pure reagents and Grade 1 water (specified in GB/T 6682) are adopted for the purpose of this method.
3.1 Reagents
3.1.1 Methanol (CH3OH).
3.1.2 Acetonitrile (CH3CN): chromatographically pure.
3.1.3 N-hexane (C6H14).
3.1.4 Zinc acetate [Zn(CH3COO)2•2H2O].
Contents of GB 22255-2014
Foreword i
1 Scope
2 Theory
3 Reagents and Materials
4 Apparatuses
5 Analytical Procedures
6 Expression of Analysis Results
7 Precision
8 Others
Annex A The Elution Program of Liquid Chromatography under Complex Conditions (Applicable to Evaporative Light-scattering Detector)
Annex B The High performance Liquid Chromatograms of Sucralose
