1 Scope
This standard specifies examination method of Yersinia enterocolitica in foods.
This standard is applicable to the examination of Yersinia enterocolitica in foods.
2 Apparatus and Materials
In addition to the conventional sterilization and cultivation equipment in microbiological laboratory, other apparatuses and materials are as follows:
2.1 Refrigerator: 0~4℃.
2.2 Constant temperature incubator: 26±1℃, 36±1℃.
2.3 Microscope: 10x~100x.
2.4 Homogenizer.
2.5 Balance: with sensibility of 0.1g.
2.6 Sterilized test tube: 16mm×160mm, 15mm×100mm.
2.7 Sterilized suction tube: 1mL (0.01mL scale), 10mL (0.1mL scale).
2.8 Conical flask: 200mL, 500mL.
2.9 Sterilized plate: 90mm in diameter.
2.10 Microbiological biochemical identification kit or system.
3 Media and Reagents
3.1 Modified phosphate buffer solution: see A.1.
3.2 CIN-1 medium (Cepulodin Irgasan Novobiocin Agar): see A.2.
3.3 Modified Y medium (Agar Y, Modified): see A.3.
3.4 Modified KIA iron medium: see A.4.
1 Scope
2 Apparatus and Materials
3 Media and Reagents
4 Examination Procedures
5 Operation Steps
6 Results and Report
Appendix A Media and Reagents
1 Scope
This standard specifies examination method of Yersinia enterocolitica in foods.
This standard is applicable to the examination of Yersinia enterocolitica in foods.
2 Apparatus and Materials
In addition to the conventional sterilization and cultivation equipment in microbiological laboratory, other apparatuses and materials are as follows:
2.1 Refrigerator: 0~4℃.
2.2 Constant temperature incubator: 26±1℃, 36±1℃.
2.3 Microscope: 10x~100x.
2.4 Homogenizer.
2.5 Balance: with sensibility of 0.1g.
2.6 Sterilized test tube: 16mm×160mm, 15mm×100mm.
2.7 Sterilized suction tube: 1mL (0.01mL scale), 10mL (0.1mL scale).
2.8 Conical flask: 200mL, 500mL.
2.9 Sterilized plate: 90mm in diameter.
2.10 Microbiological biochemical identification kit or system.
3 Media and Reagents
3.1 Modified phosphate buffer solution: see A.1.
3.2 CIN-1 medium (Cepulodin Irgasan Novobiocin Agar): see A.2.
3.3 Modified Y medium (Agar Y, Modified): see A.3.
3.4 Modified KIA iron medium: see A.4.
Contents of GB 4789.8-2016
1 Scope
2 Apparatus and Materials
3 Media and Reagents
4 Examination Procedures
5 Operation Steps
6 Results and Report
Appendix A Media and Reagents