1 Scope
This Standard specifies determination method of biotin in foods.
This Standard is applicable to determination of biotin in foods.
2 Principles
Biotin is the nutrient necessary for growth of lactobacillus plantarum. In the biotin determination culture medium, the growth of lactobacillus plantarum is linearly related to the content of biotin in the specimen to be determined, so the content of the substance to be determined in the specimen can be calculated by comparing the light transmittance and standard work curve.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Grade II water (defined in GB/T 6682) are adopted for the purposes of this method.
3.1 Reagents
3.1.1 Dehydrated alcohol (C2H6O).
3.1.2 Sodium hydroxide (NaOH).
3.1.3 Hydrochloric acid (HCl).
3.1.4 Citrate.
Contents
Foreword i
1 Scope
2 Principles
3 Reagents and Materials
4 Instruments and Apparatus
5 Preparation and Preservation of the Bacteria Strain
6 Analysis Steps
7 Precision
8 Others
Appendix A Medium and Reagents
Standard
GB 5009.259-2016 National Food Safety Standard - Determination of Biotin in Foods (English Version)
Standard No.
GB 5009.259-2016
Status
superseded
Language
English
File Format
PDF
Word Count
3500 words
Price(USD)
70.0
Implemented on
2016-9-20
Delivery
via email in 1 business day
Detail of GB 5009.259-2016
Standard No.
GB 5009.259-2016
English Name
National Food Safety Standard - Determination of Biotin in Foods
1 Scope
This Standard specifies determination method of biotin in foods.
This Standard is applicable to determination of biotin in foods.
2 Principles
Biotin is the nutrient necessary for growth of lactobacillus plantarum. In the biotin determination culture medium, the growth of lactobacillus plantarum is linearly related to the content of biotin in the specimen to be determined, so the content of the substance to be determined in the specimen can be calculated by comparing the light transmittance and standard work curve.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Grade II water (defined in GB/T 6682) are adopted for the purposes of this method.
3.1 Reagents
3.1.1 Dehydrated alcohol (C2H6O).
3.1.2 Sodium hydroxide (NaOH).
3.1.3 Hydrochloric acid (HCl).
3.1.4 Citrate.
Contents of GB 5009.259-2016
Contents
Foreword i
1 Scope
2 Principles
3 Reagents and Materials
4 Instruments and Apparatus
5 Preparation and Preservation of the Bacteria Strain
6 Analysis Steps
7 Precision
8 Others
Appendix A Medium and Reagents