1 Scope
This standard specifies determination methods of Vitamin K1 in foods.
Method I in this standard is high performance liquid chromatography-fluorescence detection method and Method II is liquid chromatography-tandem mass spectrometry, which are applicable to the determination of Vitamin K1 in all kinds of formula foods, vegetable oils, fruits and vegetables.
Method I High Performance Liquid Chromatography-Fluorescence Detection Method
2 Principle
After samples like foods for infants and young children, milk and milk products and vegetable oils are subjected to enzymolysis with lipase and amylase, Vitamin K1 in these samples is extracted by n-hexane, separated from other impurities with C18 liquid chromatographic column, subjected to zinc column reduction, detected with fluorescence detector and quantified by external standard method.
For low fat plant samples like fruit and vegetable, Vitamin K1 in them is extracted with isopropanol and n-hexane, subjected to purification by neutral alumina column to remove interfering substances like chlorophyl. Then Vitamin K1 is separated from other impurities with C18 liquid chromatographic column, subjected to zinc column reduction, detected with fluorescence detector and quantified by external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Grade I water (defined in GB/T 6682) are adopted for the purposes of this method.
3.1 Reagents
3.1.1 Absolute ethanol (CH3CH2OH).
Contents
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Apparatuses
5 Analysis Procedures
6 Expression of Analysis Results
7 Accuracy
8 Others
9 Principle
10 Reagents and Materials
11 Apparatuses
12 Analysis Procedures
13 Expression of Analysis Results
14 Accuracy
15 Others
Appendix A Calibration Method for Standard Concentration of Vitamin K
Appendix B High Performance Liquid Chromatogram
Appendix C Reference Mass Spectrogram
Standard
GB 5009.158-2016 National Food Safety Standard Determination of Vitamin K1 in Foods (English Version)
Standard No.
GB 5009.158-2016
Status
valid
Language
English
File Format
PDF
Word Count
6000 words
Price(USD)
110.0
Implemented on
2017-6-23
Delivery
via email in 1 business day
Detail of GB 5009.158-2016
Standard No.
GB 5009.158-2016
English Name
National Food Safety Standard Determination of Vitamin K1 in Foods
Chinese Name
食品安全国家标准 食品中维生素K1的测定
Chinese Classification
Professional Classification
GB
ICS Classification
Issued by
National Health and Family Planning Commission, China Food and Drug Administration
1 Scope
This standard specifies determination methods of Vitamin K1 in foods.
Method I in this standard is high performance liquid chromatography-fluorescence detection method and Method II is liquid chromatography-tandem mass spectrometry, which are applicable to the determination of Vitamin K1 in all kinds of formula foods, vegetable oils, fruits and vegetables.
Method I High Performance Liquid Chromatography-Fluorescence Detection Method
2 Principle
After samples like foods for infants and young children, milk and milk products and vegetable oils are subjected to enzymolysis with lipase and amylase, Vitamin K1 in these samples is extracted by n-hexane, separated from other impurities with C18 liquid chromatographic column, subjected to zinc column reduction, detected with fluorescence detector and quantified by external standard method.
For low fat plant samples like fruit and vegetable, Vitamin K1 in them is extracted with isopropanol and n-hexane, subjected to purification by neutral alumina column to remove interfering substances like chlorophyl. Then Vitamin K1 is separated from other impurities with C18 liquid chromatographic column, subjected to zinc column reduction, detected with fluorescence detector and quantified by external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Grade I water (defined in GB/T 6682) are adopted for the purposes of this method.
3.1 Reagents
3.1.1 Absolute ethanol (CH3CH2OH).
Contents of GB 5009.158-2016
Contents
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Apparatuses
5 Analysis Procedures
6 Expression of Analysis Results
7 Accuracy
8 Others
9 Principle
10 Reagents and Materials
11 Apparatuses
12 Analysis Procedures
13 Expression of Analysis Results
14 Accuracy
15 Others
Appendix A Calibration Method for Standard Concentration of Vitamin K
Appendix B High Performance Liquid Chromatogram
Appendix C Reference Mass Spectrogram