National Food Safety Standard-Determination of Malondialdehyde in Foods
1 Scope
This standard specifies the determination method of malondialdehyde in foods.
For the purposes of this standard, Method I is applicable to the determination of malondialdehyde in foods and Method II is applicable to the determination of malondialdehyde in animal and vegetable fats and oils.
Method I High Performance Liquid Chromatography
2 Principle
Extract the specimen with acid solution, then the extract and thiobarbituric acid react to generate colored compound, determine by high performance liquid chromatography - diode array detector and carry out quantitative determination by external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Class-I water (defined in GB/T 6682) are adopted for the purpose of this method.
3.1 Reagents
3.1.1 Methanol (CH3OH): chromatographically pure.
3.1.2 Trichloroacetic acid (C2HCl3O2).
3.1.3 Ethylenediamine tetraacetic acid disodium (C10H14N2Na2O8·2H2O).
3.1.4 Thiobarbituric acid (TBA) (C4H4N2O2S).
3.2 Preparation of reagents
3.2.1 Ammonium acetate solution (0.01 mol/L): weigh 0.77 g ammonium acetate, dissolve it with water, scale the volume to 1,000 mL and filter with 0.45 μm filter.
3.2.2 Trichloroacetic acid solution: accurately weigh 37.50 g (accurate to 0.01 g) trichloroacetic acid (3.1.2) and 0.50 g (accurate to 0.01 g) ethylenediamine tetraacetic acid disodium (3.1.3), dissolve in water and dilute to 500 mL.
3.2.3 Thiobarbituric acid (TBA) solution: accurately weigh 0.288 g (accurate to 0.001 g) thiobarbituric acid (3.1.4), dissolve in water and dilute to 100 mL (if it is not easy to dissolve, carry out ultrasonic heating till complete dissolution and scale the volume to 100 mL after cooling), equivalent to 0.02 mol/L.
3.3 Standard product
1,1,3,3-tetraethoxypropane (C11H24O4, CAS No.: 122-31-6): purity ≥ 97%.
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Steps
6 Expression of Analysis Results
7 Accuracy
8 Principle
9 Reagents and Materials
10 Instruments and Apparatus
11 Analysis Steps
12 Expression of Analysis Results
13 Accuracy
14 Other
Standard
GB 5009.181-2016 National Food Safety Standard - Determination of malondialdehyde in food (English Version)
Standard No.
GB 5009.181-2016
Status
valid
Language
English
File Format
PDF
Word Count
2000 words
Price(USD)
60.0
Implemented on
2017-1-1
Delivery
via email in 1 business day
Detail of GB 5009.181-2016
Standard No.
GB 5009.181-2016
English Name
National Food Safety Standard - Determination of malondialdehyde in food
National Food Safety Standard-Determination of Malondialdehyde in Foods
1 Scope
This standard specifies the determination method of malondialdehyde in foods.
For the purposes of this standard, Method I is applicable to the determination of malondialdehyde in foods and Method II is applicable to the determination of malondialdehyde in animal and vegetable fats and oils.
Method I High Performance Liquid Chromatography
2 Principle
Extract the specimen with acid solution, then the extract and thiobarbituric acid react to generate colored compound, determine by high performance liquid chromatography - diode array detector and carry out quantitative determination by external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Class-I water (defined in GB/T 6682) are adopted for the purpose of this method.
3.1 Reagents
3.1.1 Methanol (CH3OH): chromatographically pure.
3.1.2 Trichloroacetic acid (C2HCl3O2).
3.1.3 Ethylenediamine tetraacetic acid disodium (C10H14N2Na2O8·2H2O).
3.1.4 Thiobarbituric acid (TBA) (C4H4N2O2S).
3.2 Preparation of reagents
3.2.1 Ammonium acetate solution (0.01 mol/L): weigh 0.77 g ammonium acetate, dissolve it with water, scale the volume to 1,000 mL and filter with 0.45 μm filter.
3.2.2 Trichloroacetic acid solution: accurately weigh 37.50 g (accurate to 0.01 g) trichloroacetic acid (3.1.2) and 0.50 g (accurate to 0.01 g) ethylenediamine tetraacetic acid disodium (3.1.3), dissolve in water and dilute to 500 mL.
3.2.3 Thiobarbituric acid (TBA) solution: accurately weigh 0.288 g (accurate to 0.001 g) thiobarbituric acid (3.1.4), dissolve in water and dilute to 100 mL (if it is not easy to dissolve, carry out ultrasonic heating till complete dissolution and scale the volume to 100 mL after cooling), equivalent to 0.02 mol/L.
3.3 Standard product
1,1,3,3-tetraethoxypropane (C11H24O4, CAS No.: 122-31-6): purity ≥ 97%.
Contents of GB 5009.181-2016
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Steps
6 Expression of Analysis Results
7 Accuracy
8 Principle
9 Reagents and Materials
10 Instruments and Apparatus
11 Analysis Steps
12 Expression of Analysis Results
13 Accuracy
14 Other