1 Scope
This standard specifies the examination method for Listeria monocytogenes in food.
In this standard, Method I is applicable to the qualitative examination of Listeria monocytogenes in food; Method II is applicable to enumeration of the Listeria monocytogenes in food with high content of Listeria monocytogenes; Method III is applicable to enumeration of the Listeria monocytogenes in food with low content of Listeria monocytogenes (<100CFU/g) but high content of infectious microbe, especially the milk, water and food containing particulate matters that interfere with colony counting.
2 Apparatus and Materials
In addition to the apparatus for conventional sterilization and cultivation in microbiological laboratory, other apparatus and materials are as follows:
2.1 Refrigerator: 2℃~5℃.
2.2 Constant temperature incubator: 30℃±1℃, 36℃±1℃.
2.3 Homogenizer.
2.4 Microscope: 10x~100x.
2.5 Electronic balance: 0.1g in sensibility.
2.6 Conical flask: 100mL, 500mL.
2.7 Aseptic pipette: 1mL (scale division of 0.01mL), 10mL (scale division of 0.1mL) or micropipettor and pipette tip.
Contents
Foreword i
1 Scope
2 Apparatus and Materials
3 Culture Medias and Reagents
4 Examination Procedures
5 Operation Steps
6 Examination Procedures
7 Operation Steps
8 Result Enumeration
9 Result Report
10 Examination Procedures
11 Operation Steps
12 Result and Report
Appendix A Media and Reagent
Appendix B Most Probable Number (MPN) of Listeria Monocytogenes
Standard
GB 4789.30-2016 National Food Safety Standard—Food Microbiological Examination—Examination of Listeria Monocytogenes (English Version)
Standard No.
GB 4789.30-2016
Status
valid
Language
English
File Format
PDF
Word Count
6000 words
Price(USD)
110.0
Implemented on
2017-6-23
Delivery
via email in 1 business day
Detail of GB 4789.30-2016
Standard No.
GB 4789.30-2016
English Name
National Food Safety Standard—Food Microbiological Examination—Examination of Listeria Monocytogenes
Chinese Name
食品安全国家标准 食品微生物学检验 单核细胞增生李斯特氏菌检验
Chinese Classification
Professional Classification
GB
ICS Classification
Issued by
National Health and Family Planning Commission, China Food and Drug Administration
1 Scope
This standard specifies the examination method for Listeria monocytogenes in food.
In this standard, Method I is applicable to the qualitative examination of Listeria monocytogenes in food; Method II is applicable to enumeration of the Listeria monocytogenes in food with high content of Listeria monocytogenes; Method III is applicable to enumeration of the Listeria monocytogenes in food with low content of Listeria monocytogenes (<100CFU/g) but high content of infectious microbe, especially the milk, water and food containing particulate matters that interfere with colony counting.
2 Apparatus and Materials
In addition to the apparatus for conventional sterilization and cultivation in microbiological laboratory, other apparatus and materials are as follows:
2.1 Refrigerator: 2℃~5℃.
2.2 Constant temperature incubator: 30℃±1℃, 36℃±1℃.
2.3 Homogenizer.
2.4 Microscope: 10x~100x.
2.5 Electronic balance: 0.1g in sensibility.
2.6 Conical flask: 100mL, 500mL.
2.7 Aseptic pipette: 1mL (scale division of 0.01mL), 10mL (scale division of 0.1mL) or micropipettor and pipette tip.
Contents of GB 4789.30-2016
Contents
Foreword i
1 Scope
2 Apparatus and Materials
3 Culture Medias and Reagents
4 Examination Procedures
5 Operation Steps
6 Examination Procedures
7 Operation Steps
8 Result Enumeration
9 Result Report
10 Examination Procedures
11 Operation Steps
12 Result and Report
Appendix A Media and Reagent
Appendix B Most Probable Number (MPN) of Listeria Monocytogenes