1 Scope
This standard specifies the method for examination of staphylococcus aureus in foods.
In this standard, Method I is applicable to qualitative examination of staphylococcus aureus in foods, Method II to enumeration of staphylococcus aureus in foods containing more staphylococcus aureus and Method III to enumeration of staphylococcus aureus in foods containing fewer staphylococcus aureus.
2 Apparatus and Materials
In addition to the apparatus for conventional sterilization and cultivation in microbiological laboratory, other apparatus and materials are as follows:
2.1 Constant temperature incubator: 36℃±1℃.
2.2 Refrigerator: 2℃~5℃.
2.3 Thermostatic water bath: 36℃~56℃.
2.4 Balance: with sensibility of 0.1g.
2.5 Homogenizer.
2.6 Oscillator.
2.7 Aseptic pipette: 1mL (with scale division of 0.01mL), 10mL (with scale division of 0.1mL) or micropipettor and pipette tips.
2.8 Aseptic conical flask: 100mL and 500mL.
2.9 Aseptic culture dish: 90mm in diameter.
2.10 Coating rod.
2.11 pH meter or pH colorimetric tube or precise pH paper.
3 Culture Media and Reagents
3.1 7.5% sodium chloride broth: see A.1.
3.2 Blood agar plate: see A.2.
3.3 Baird-Parker agar plate: see A.3.
3.4 Brain heart infusion broth (BHI): see A.4.
Contents
Foreword i
1 Scope
2 Apparatus and Materials
3 Culture Media and Reagents
Method I Qualitative Examination of Staphylococcus Aureus
4 Examination Procedures
5 Operation Steps
6 Result and Report
Method II Plate Counting Method of Staphylococcus Aureus
7 Examination Procedures
8 Operation Steps
9 Calculation of Result
10 Report
Method III MPN Enumeration of Staphylococcus Aureus
11 Examination Procedures
12 Operation Steps
13 Result and Report
Appendix A Culture Media and Reagents
Appendix B Examination of Staphylococcal Enterotoxin
Appendix C MPN Retrieval Table of Staphylococcus Aureus
1 Scope
This standard specifies the method for examination of staphylococcus aureus in foods.
In this standard, Method I is applicable to qualitative examination of staphylococcus aureus in foods, Method II to enumeration of staphylococcus aureus in foods containing more staphylococcus aureus and Method III to enumeration of staphylococcus aureus in foods containing fewer staphylococcus aureus.
2 Apparatus and Materials
In addition to the apparatus for conventional sterilization and cultivation in microbiological laboratory, other apparatus and materials are as follows:
2.1 Constant temperature incubator: 36℃±1℃.
2.2 Refrigerator: 2℃~5℃.
2.3 Thermostatic water bath: 36℃~56℃.
2.4 Balance: with sensibility of 0.1g.
2.5 Homogenizer.
2.6 Oscillator.
2.7 Aseptic pipette: 1mL (with scale division of 0.01mL), 10mL (with scale division of 0.1mL) or micropipettor and pipette tips.
2.8 Aseptic conical flask: 100mL and 500mL.
2.9 Aseptic culture dish: 90mm in diameter.
2.10 Coating rod.
2.11 pH meter or pH colorimetric tube or precise pH paper.
3 Culture Media and Reagents
3.1 7.5% sodium chloride broth: see A.1.
3.2 Blood agar plate: see A.2.
3.3 Baird-Parker agar plate: see A.3.
3.4 Brain heart infusion broth (BHI): see A.4.
Contents of GB 4789.10-2016
Contents
Foreword i
1 Scope
2 Apparatus and Materials
3 Culture Media and Reagents
Method I Qualitative Examination of Staphylococcus Aureus
4 Examination Procedures
5 Operation Steps
6 Result and Report
Method II Plate Counting Method of Staphylococcus Aureus
7 Examination Procedures
8 Operation Steps
9 Calculation of Result
10 Report
Method III MPN Enumeration of Staphylococcus Aureus
11 Examination Procedures
12 Operation Steps
13 Result and Report
Appendix A Culture Media and Reagents
Appendix B Examination of Staphylococcal Enterotoxin
Appendix C MPN Retrieval Table of Staphylococcus Aureus