1 Scope
This standard specifies the examination method of clostridium botulinum and botulinum toxin in foods.
This standard is applicable to the examination of clostridium botulinum and botulinum toxin in foods.
2 Apparatus and Materials
In addition to the apparatus for conventional sterilization and cultivation in microbiological laboratory, other apparatus and materials are as follows:
2.1 Refrigerator: 2℃~5℃, -20℃.
2.2 Balance: with sensibility of 0.1g.
2.3 Aseptic surgical scissors, tweezers and reagent scoop.
2.4 Homogenizer or aseptic mortar.
2.5 Centrifuge: 3 000r/min, 14 000r/min.
2.6 Anaerobic culture apparatus.
2.7 Constant temperature incubator: 35℃±1℃, 28℃±1℃.
2.8 Thermostatic water bath: 37℃±1℃, 60℃±1℃, 80℃±1℃.
2.9 Microscope: 10x~100x.
2.10 PCR instrument.
2.11 Electrophoresis apparatus or capillary electrophoresis apparatus.
2.12 Gel imaging system or ultraviolet detector.
2.13 Nucleic acid protein analyzer or ultraviolet spectrophotometer.
2.14 Adjustable micropipettor: 0.2μL~2μL, 2μL~20μL, 20μL~200μL, 100μL~1 000μL.
2.15 Aseptic pipette: 1.0mL, 10.0mL, 25.0mL.
2.16 Aseptic conical flask: 100mL.
2.17 Culture dish: 90mm in diameter.
2.18 Centrifugal tube: 50mL, 1.5mL.
2.19 PCR reaction tube.
2.20 Aseptic injector: 1.0mL.
2.21 Mice: 15g~20g, the KM or ICR mice with same strain shall be adopted for each batch of test.
3 Media and Reagents
Contents
Foreword I
1 Scope
2 Apparatus and Materials
3 Media and Reagents
4 Examination Procedures
5 Operation Steps
6 Result Report
Appendix A Media and Reagents
1 Scope
This standard specifies the examination method of clostridium botulinum and botulinum toxin in foods.
This standard is applicable to the examination of clostridium botulinum and botulinum toxin in foods.
2 Apparatus and Materials
In addition to the apparatus for conventional sterilization and cultivation in microbiological laboratory, other apparatus and materials are as follows:
2.1 Refrigerator: 2℃~5℃, -20℃.
2.2 Balance: with sensibility of 0.1g.
2.3 Aseptic surgical scissors, tweezers and reagent scoop.
2.4 Homogenizer or aseptic mortar.
2.5 Centrifuge: 3 000r/min, 14 000r/min.
2.6 Anaerobic culture apparatus.
2.7 Constant temperature incubator: 35℃±1℃, 28℃±1℃.
2.8 Thermostatic water bath: 37℃±1℃, 60℃±1℃, 80℃±1℃.
2.9 Microscope: 10x~100x.
2.10 PCR instrument.
2.11 Electrophoresis apparatus or capillary electrophoresis apparatus.
2.12 Gel imaging system or ultraviolet detector.
2.13 Nucleic acid protein analyzer or ultraviolet spectrophotometer.
2.14 Adjustable micropipettor: 0.2μL~2μL, 2μL~20μL, 20μL~200μL, 100μL~1 000μL.
2.15 Aseptic pipette: 1.0mL, 10.0mL, 25.0mL.
2.16 Aseptic conical flask: 100mL.
2.17 Culture dish: 90mm in diameter.
2.18 Centrifugal tube: 50mL, 1.5mL.
2.19 PCR reaction tube.
2.20 Aseptic injector: 1.0mL.
2.21 Mice: 15g~20g, the KM or ICR mice with same strain shall be adopted for each batch of test.
3 Media and Reagents
Contents of GB 4789.12-2016
Contents
Foreword I
1 Scope
2 Apparatus and Materials
3 Media and Reagents
4 Examination Procedures
5 Operation Steps
6 Result Report
Appendix A Media and Reagents
