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GB 4789.15-2016   National Food Safety Standard---Food Microbiological Examination: Enumeration of Moulds and Yeasts (English Version)
Standard No.: GB 4789.15-2016 Status:valid remind me the status change

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Language:English File Format:PDF
Word Count: 3000 words Price(USD):60.0 remind me the price change

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Implemented on:2017-4-19 Delivery: via email in 1 business day
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Standard No.: GB 4789.15-2016
English Name: National Food Safety Standard---Food Microbiological Examination: Enumeration of Moulds and Yeasts
Chinese Name: 食品安全国家标准 食品微生物学检验 霉菌和酵母计数
Professional Classification: GB    National Standard
Issued by: National Health and Family Planning Commission
Issued on: 2016-10-19
Implemented on: 2017-4-19
Status: valid
Superseding:GB 4789.15-2010 Food Microbiological Examination: Enumeration of Moulds and Yeasts
SN/T 2552.3-2010 Microbiological Examination Method for Milk and Milk Products Hygiene - Part 3: Colony-count Method of Yeast and Moulds
Language: English
File Format: PDF
Word Count: 3000 words
Price(USD): 60.0
Delivery: via email in 1 business day
This standard replaces GB 4789.15-2010 National Food Safety Standard Food Microbiological Examination: Enumeration of Moulds and Yeasts and SN/T 2552.3-2010 Microbiological Examination for Milk and Milk Products Hygiene - Part 3: Colony-count Method of Yeast and Moulds. Compared with GB 4789.15-2010, the main changes in this standard are as follows: - The Chapter "Apparatus and Materials" is modified; - The Chapter "Culture Media and Reagents" is modified; - The Chapters of “Examination Procedures” and “Operation Steps” are modified; - The Chapter “Result and Report” is modified; - Appendix A is modified; - Appendix B is modified as Method II. National food safety standard Food microbiological examination: Enumeration of moulds and yeasts 1 Scope This standard specifies the enumeration methods of moulds and yeasts in foods. In this standard, Method I is applicable to the enumeration of molds and yeasts in various foods while Method II is applicable to that of moulds in canned tomato sauce and tomato juice. 2 Apparatus and Materials In addition to the conventional sterilization and cultivation apparatus in microbiological laboratory, other apparatus and materials are as follows: 2.1 Incubator: 28±1℃. 2.2 Slap type homogenizer and homogenizing bag. 2.3 Electronic balance: with sensibility of 0.1g. 2.4 Aseptic conical flask: with volume of 500mL. 2.5 Aseptic suction tube: 1mL (with scale division of 0.01mL) and 10mL (with scale division of 0.1mL). 2.6 Aseptic test tube: 18×180 mm. 2.7 Vortex mixers. 2.8 Aseptic plate: with diameter of 90mm. 2.9 Thermostatic water bath: 46±1℃. 2.10 Microscope: 10×~100×. 2.11 Micropipettor and tip: 1.0mL. 2.12 Refractometer. 2.13 Howard measuring slide: special side with standard measuring chamber. 2.14 Cover glass. 2.15 Micrometer: slide with standard scale. 3 Culture Media and Reagents 3.1 Normal saline: see A.1. 3.2 Potato dextrose agar: see A.2. 3.3 Rose bengal agar: see A.3. 3.4 Phosphate buffer solution: see A.4. Method I: Enumeration of Molds and Yeasts by Plate Count 4 Examination Procedure Examination procedures for the enumeration of molds and yeasts by plate count are shown in Figure 1. Figure 1 Examination Procedures for the Enumeration of Molds and Yeasts by Plate Count 5 Operation Steps 5.1 Dilution of sample 5.1.1 Solid and semi-solid sample: weigh 25g sample, add 225mL aseptic diluent (distilled water/normal saline/phosphate buffer solution) and shake well or slap for 1~2min with slap type homogenizer to prepare 1:10 homogeneous sample solution. 5.1.2 Liquid sample: pipet 25mL sample with aseptic suction tube and put into an appropriate container (proper number of aseptic glass beads may be preset in the flask) or aseptic homogenizing bag with 225mL aseptic diluent (distilled water/normal saline/phosphate buffer solution), shake the container well or slap for 1~2min with slap type homogenizer to prepare 1:10 homogeneous sample solution. 5.1.3 Pipet 1mL 1:10 homogeneous sample solution and inject into the test tube with 9mL aseptic diluent, use another 1mL aseptic suction tube to blow and suck repeatedly or mix it on vortex mixer so that it is mixed uniformly, thus such solution is the 1:100 homogeneous sample solution. 5.1.4 According to the operation procedures stated in 5.1.3, prepare 10-fold diluted homogeneous sample solution successively. Per increasing dilution, use another 1mL aseptic suction tube. 5.1.5 According to the estimation for the sample contamination status, select 2~3 portions of homogeneous sample solutions (stock solution may be selected as the liquid sample) with appropriate dilutions; during the 10-fold increasing dilution, pipet 1 mL homogeneous sample solution for each dilution respectively and then add them into two sterile plates. Meanwhile, take 1mL aseptic diluent and put into two sterile plates as blank control. 5.1.6 Pour 20~25mL potato dextrose agar or rose bengal agar cooled to 46℃ (it may be put in 46±1℃ thermostatic water bath for thermal insulation) into a plate timely, and rotate the plate to mix them uniformly. Place the plate on the horizontal stand until the culture medium is completely solidified. 5.2 Culture After the agar is solidified, turn the plate, cultivate it in 28±1℃ incubator and observe and record the result on the 5th day. 5.3 Colony count Carry out visual observation, if necessary, by magnifier or low power lens, and record the dilution times and the corresponding numbers of colonies in moulds and yeasts. The count is expressed in colony-forming units (CFU). Select the plate with the colony number of 10~150 CFU is selected, and enumerate the moulds and yeasts respectively according to the colonial morphology. If the moulds spread and cover the whole plate, it may be recorded as colony spreading.
Foreword I 1 Scope 2 Apparatus and Materials 3 Culture Media and Reagents 4 Examination Procedure 5 Operation Steps 6 Result and Report 7 Operation Steps Appendix A Culture Media and Reagents
Referred in GB 4789.15-2016:
*GB 4806.11-2016 National Food Safety Standard -- Food Contact Rubber Materials and Articles
*GB 4806.10-2016 National Food Safety Standard-Food Contact Paints and Coatings
*GB 4806.9-2016 National Food Safety Standard -Food Contact Metal Materials and Articles
*GB 4806.8-2016 National Food Safety Standard-Food Contact Paper and Board Materials and Their Products
*GB 4806.7-2016 National Food Safety Standard - Food Contact Plastic Materials and Articles
*GB 4806.6-2016 National Food Safety Standard-Plastic Resin used in Food-contact
*GB 4806.5-2016 National Food Safety Standard-Glass Products
*YD/T 993-2016 The technical requirements and test methods of overvoltages and overcurrents resistibility for wire telecommunication terminal equipment
*GB 4806.3-2016 National Food Safety Standard-Enamelware
*GB 4806.1-2016 National Food Safety Standard-General Safety Requirements on Food Contact Materials and Articles
*GB 4806.4-2016 National Food Safety Standard- Ceramic ware
*GB/T 20564.9-2016 Continuously cold rolled high strength steel sheet and strip for automobile一Part 9:Quenching and partitfoning steel
*YD/T 2687-2013/XG1-2016 Technical requirements and test methods for multimode user equipment(Single card slot)of LTE/CDMA, includes Amendment 1
*CQC 1105-2015 Technical Specification for Cables of Electric Vehicles Conductive Charging Systems Part 3: Cables for DC Charging Systems
GB 4789.15-2016 is referred in:
*GB 14934-1994 Hygienic standard for disinfection of dinner and drinking set
*NY/T 1039-2014 Green food - Starch and starch product
*QB/T 5426-2019 Household and similar electrical steam mop
*GB/T 20886.1-2021 Quality requirements for yeast products—Part 1:Yeast for food processing
*TCAQI 87-2019 Technical requirements and evaluative methods for storage function of dishwasher
*QB/T 1520-2023 Household and similar electrical dishwasher
*FZ/T 01137-2016 Textiles- Determination of fluorescent whitening agents
*FZ/T 01028-2016 Textiles―Burning behaviour―Determination of flame spread rate of horizontally oriented specimens
*GB 5009.156-2016 National Food Safety Standard--General Principle of Migration Test Pre-treatment Method of Food Contact Materials and Their Products
*QB/T 4575-2023 Microbial food cultures preparations
*GB/T 21551.2-2024 Antimicrobial and cleaning function of household and similar electrical appliances—Part 2: Particular requirements for material
*GB/T 21551.3-2024 Antimicrobial and cleaning function of household and similar electrical appliances—Part 3 : Particular requirements for air cleaner
Code of China
Standard
GB 4789.15-2016  National Food Safety Standard---Food Microbiological Examination: Enumeration of Moulds and Yeasts (English Version)
Standard No.GB 4789.15-2016
Statusvalid
LanguageEnglish
File FormatPDF
Word Count3000 words
Price(USD)60.0
Implemented on2017-4-19
Deliveryvia email in 1 business day
Detail of GB 4789.15-2016
Standard No.
GB 4789.15-2016
English Name
National Food Safety Standard---Food Microbiological Examination: Enumeration of Moulds and Yeasts
Chinese Name
食品安全国家标准 食品微生物学检验 霉菌和酵母计数
Chinese Classification
Professional Classification
GB
ICS Classification
Issued by
National Health and Family Planning Commission
Issued on
2016-10-19
Implemented on
2017-4-19
Status
valid
Superseded by
Superseded on
Abolished on
Superseding
GB 4789.15-2010 Food Microbiological Examination: Enumeration of Moulds and Yeasts
SN/T 2552.3-2010 Microbiological Examination Method for Milk and Milk Products Hygiene - Part 3: Colony-count Method of Yeast and Moulds
Language
English
File Format
PDF
Word Count
3000 words
Price(USD)
60.0
Keywords
GB 4789.15-2016, GB/T 4789.15-2016, GBT 4789.15-2016, GB4789.15-2016, GB 4789.15, GB4789.15, GB/T4789.15-2016, GB/T 4789.15, GB/T4789.15, GBT4789.15-2016, GBT 4789.15, GBT4789.15
Introduction of GB 4789.15-2016
This standard replaces GB 4789.15-2010 National Food Safety Standard Food Microbiological Examination: Enumeration of Moulds and Yeasts and SN/T 2552.3-2010 Microbiological Examination for Milk and Milk Products Hygiene - Part 3: Colony-count Method of Yeast and Moulds. Compared with GB 4789.15-2010, the main changes in this standard are as follows: - The Chapter "Apparatus and Materials" is modified; - The Chapter "Culture Media and Reagents" is modified; - The Chapters of “Examination Procedures” and “Operation Steps” are modified; - The Chapter “Result and Report” is modified; - Appendix A is modified; - Appendix B is modified as Method II. National food safety standard Food microbiological examination: Enumeration of moulds and yeasts 1 Scope This standard specifies the enumeration methods of moulds and yeasts in foods. In this standard, Method I is applicable to the enumeration of molds and yeasts in various foods while Method II is applicable to that of moulds in canned tomato sauce and tomato juice. 2 Apparatus and Materials In addition to the conventional sterilization and cultivation apparatus in microbiological laboratory, other apparatus and materials are as follows: 2.1 Incubator: 28±1℃. 2.2 Slap type homogenizer and homogenizing bag. 2.3 Electronic balance: with sensibility of 0.1g. 2.4 Aseptic conical flask: with volume of 500mL. 2.5 Aseptic suction tube: 1mL (with scale division of 0.01mL) and 10mL (with scale division of 0.1mL). 2.6 Aseptic test tube: 18×180 mm. 2.7 Vortex mixers. 2.8 Aseptic plate: with diameter of 90mm. 2.9 Thermostatic water bath: 46±1℃. 2.10 Microscope: 10×~100×. 2.11 Micropipettor and tip: 1.0mL. 2.12 Refractometer. 2.13 Howard measuring slide: special side with standard measuring chamber. 2.14 Cover glass. 2.15 Micrometer: slide with standard scale. 3 Culture Media and Reagents 3.1 Normal saline: see A.1. 3.2 Potato dextrose agar: see A.2. 3.3 Rose bengal agar: see A.3. 3.4 Phosphate buffer solution: see A.4. Method I: Enumeration of Molds and Yeasts by Plate Count 4 Examination Procedure Examination procedures for the enumeration of molds and yeasts by plate count are shown in Figure 1. Figure 1 Examination Procedures for the Enumeration of Molds and Yeasts by Plate Count 5 Operation Steps 5.1 Dilution of sample 5.1.1 Solid and semi-solid sample: weigh 25g sample, add 225mL aseptic diluent (distilled water/normal saline/phosphate buffer solution) and shake well or slap for 1~2min with slap type homogenizer to prepare 1:10 homogeneous sample solution. 5.1.2 Liquid sample: pipet 25mL sample with aseptic suction tube and put into an appropriate container (proper number of aseptic glass beads may be preset in the flask) or aseptic homogenizing bag with 225mL aseptic diluent (distilled water/normal saline/phosphate buffer solution), shake the container well or slap for 1~2min with slap type homogenizer to prepare 1:10 homogeneous sample solution. 5.1.3 Pipet 1mL 1:10 homogeneous sample solution and inject into the test tube with 9mL aseptic diluent, use another 1mL aseptic suction tube to blow and suck repeatedly or mix it on vortex mixer so that it is mixed uniformly, thus such solution is the 1:100 homogeneous sample solution. 5.1.4 According to the operation procedures stated in 5.1.3, prepare 10-fold diluted homogeneous sample solution successively. Per increasing dilution, use another 1mL aseptic suction tube. 5.1.5 According to the estimation for the sample contamination status, select 2~3 portions of homogeneous sample solutions (stock solution may be selected as the liquid sample) with appropriate dilutions; during the 10-fold increasing dilution, pipet 1 mL homogeneous sample solution for each dilution respectively and then add them into two sterile plates. Meanwhile, take 1mL aseptic diluent and put into two sterile plates as blank control. 5.1.6 Pour 20~25mL potato dextrose agar or rose bengal agar cooled to 46℃ (it may be put in 46±1℃ thermostatic water bath for thermal insulation) into a plate timely, and rotate the plate to mix them uniformly. Place the plate on the horizontal stand until the culture medium is completely solidified. 5.2 Culture After the agar is solidified, turn the plate, cultivate it in 28±1℃ incubator and observe and record the result on the 5th day. 5.3 Colony count Carry out visual observation, if necessary, by magnifier or low power lens, and record the dilution times and the corresponding numbers of colonies in moulds and yeasts. The count is expressed in colony-forming units (CFU). Select the plate with the colony number of 10~150 CFU is selected, and enumerate the moulds and yeasts respectively according to the colonial morphology. If the moulds spread and cover the whole plate, it may be recorded as colony spreading.
Contents of GB 4789.15-2016
Foreword I 1 Scope 2 Apparatus and Materials 3 Culture Media and Reagents 4 Examination Procedure 5 Operation Steps 6 Result and Report 7 Operation Steps Appendix A Culture Media and Reagents
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