1 Scope
This standard specifies the enumeration methods of moulds and yeasts in foods.
In this standard, Method I is applicable to the enumeration of molds and yeasts in various foods while Method II is applicable to that of moulds in canned tomato sauce and tomato juice.
2 Apparatus and Materials
In addition to the conventional sterilization and cultivation apparatus in microbiological laboratory, other apparatus and materials are as follows:
2.1 Incubator: 28±1℃.
2.2 Slap type homogenizer and homogenizing bag.
2.3 Electronic balance: with sensibility of 0.1g.
2.4 Aseptic conical flask: with volume of 500mL.
2.5 Aseptic suction tube: 1mL (with scale division of 0.01mL) and 10mL (with scale division of 0.1mL).
2.6 Aseptic test tube: 18×180 mm.
2.7 Vortex mixers.
2.8 Aseptic plate: with diameter of 90mm.
2.9 Thermostatic water bath: 46±1℃.
2.10 Microscope: 10×~100×.
2.11 Micropipettor and tip: 1.0mL.
2.12 Refractometer.
2.13 Howard measuring slide: special side with standard measuring chamber.
2.14 Cover glass.
2.15 Micrometer: slide with standard scale.
3 Culture Media and Reagents
3.1 Normal saline: see A.1.
3.2 Potato dextrose agar: see A.2.
3.3 Rose bengal agar: see A.3.
3.4 Phosphate buffer solution: see A.4.
Method I: Enumeration of Molds and Yeasts by Plate Count
Foreword I
1 Scope
2 Apparatus and Materials
3 Culture Media and Reagents
4 Examination Procedure
5 Operation Steps
6 Result and Report
7 Operation Steps
Appendix A Culture Media and Reagents
1 Scope
This standard specifies the enumeration methods of moulds and yeasts in foods.
In this standard, Method I is applicable to the enumeration of molds and yeasts in various foods while Method II is applicable to that of moulds in canned tomato sauce and tomato juice.
2 Apparatus and Materials
In addition to the conventional sterilization and cultivation apparatus in microbiological laboratory, other apparatus and materials are as follows:
2.1 Incubator: 28±1℃.
2.2 Slap type homogenizer and homogenizing bag.
2.3 Electronic balance: with sensibility of 0.1g.
2.4 Aseptic conical flask: with volume of 500mL.
2.5 Aseptic suction tube: 1mL (with scale division of 0.01mL) and 10mL (with scale division of 0.1mL).
2.6 Aseptic test tube: 18×180 mm.
2.7 Vortex mixers.
2.8 Aseptic plate: with diameter of 90mm.
2.9 Thermostatic water bath: 46±1℃.
2.10 Microscope: 10×~100×.
2.11 Micropipettor and tip: 1.0mL.
2.12 Refractometer.
2.13 Howard measuring slide: special side with standard measuring chamber.
2.14 Cover glass.
2.15 Micrometer: slide with standard scale.
3 Culture Media and Reagents
3.1 Normal saline: see A.1.
3.2 Potato dextrose agar: see A.2.
3.3 Rose bengal agar: see A.3.
3.4 Phosphate buffer solution: see A.4.
Method I: Enumeration of Molds and Yeasts by Plate Count
Contents of GB 4789.15-2016
Foreword I
1 Scope
2 Apparatus and Materials
3 Culture Media and Reagents
4 Examination Procedure
5 Operation Steps
6 Result and Report
7 Operation Steps
Appendix A Culture Media and Reagents
